Department of Respiratory Medicine, Huangdao Division, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Sep;21(18):4113-4123.
Lung cancer is the most common malignancy with the highest mortality rate among cancers. microRNAs (miRNAs) have been confirmed to be closely related to the physiological disorder, especially the tumor process. This study aimed to investigate the effect of miR-27b-3p on lung tumor cells.
The expressions of miR-27b-3p in lung tumors and adjacent non-tumors lung tissues were compared. We test the bonding effect of miR-27b-3p on the Fzd7 promoter, and miR-27b-3p effects on the Fzd7 expression in both NCI-H446 and A549 cells. Then, effects of miR-27b-3p and Fzd7 on these cells viability, survival and apoptosis were detected, respectively. In addition, the possible mechanism of miR-27b-3p affected these cells apoptosis was explored by analyzing the expression of apoptosis-related factors.
We found that miR-27b-3p was low expressed in lung tumors compared to adjacent non-tumorous lung tissues. miR-27b-3p directly targeted Fzd7 promoter and negatively regulated Fzd7 expression. Fzd7 promoted NCI-H446 and A549 cells viability and survival, inhibited cells apoptosis. However, miR-27b-3p effects on these cells were quite the opposite to Fzd7. The expressions of apoptosis-related factors were associated positively with miR-27b-3p and showed a negative correlation with Fzd7 expression.
The miR-27b-3p was lowly expressed in lung cancer tissues, and played the role of a tumor suppressor. It could promote cell apoptosis and suppress cancer cells viability and survival via down-regulating Fzd7. It suggested that miR-27b-3p might be a potential target for the prophylaxis and treatment of lung cancer.
肺癌是癌症中最常见的恶性肿瘤，死亡率最高。microRNAs（miRNAs）已被证实与生理紊乱密切相关，尤其是肿瘤过程。本研究旨在探讨 miR-27b-3p 对肺肿瘤细胞的影响。
比较肺肿瘤和相邻非肿瘤肺组织中 miR-27b-3p 的表达。我们测试了 miR-27b-3p 对 Fzd7 启动子的结合效应，以及 miR-27b-3p 对 NCI-H446 和 A549 细胞中 Fzd7 表达的影响。然后，分别检测 miR-27b-3p 和 Fzd7 对这些细胞活力、存活和凋亡的影响。此外，通过分析凋亡相关因子的表达，探讨了 miR-27b-3p 影响这些细胞凋亡的可能机制。
我们发现 miR-27b-3p 在肺肿瘤中的表达低于相邻的非肿瘤肺组织。miR-27b-3p 直接靶向 Fzd7 启动子并负调控 Fzd7 表达。Fzd7 促进 NCI-H446 和 A549 细胞活力和存活，抑制细胞凋亡。然而，miR-27b-3p 对这些细胞的作用与 Fzd7 完全相反。凋亡相关因子的表达与 miR-27b-3p 呈正相关，与 Fzd7 表达呈负相关。
miR-27b-3p 在肺癌组织中低表达，发挥肿瘤抑制作用。它可以通过下调 Fzd7 促进细胞凋亡，抑制癌细胞活力和存活。这表明 miR-27b-3p 可能是预防和治疗肺癌的潜在靶点。
