Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
Research Unit 'Perioperative Platelet Function', Medical University of Graz, Graz, Austria.
J Thromb Haemost. 2017 Dec;15(12):2443-2450. doi: 10.1111/jth.13870. Epub 2017 Nov 22.
Essentials In platelet function testing, standardized internal controls (IQC) are not commercially provided. Platelet function testing was performed daily on aliquoted pooled platelet concentrates. Pooled platelet concentrates showed stability for control purposes from Monday to Friday. Pooled platelet concentrates provide the necessary steadiness to serve as IQC material.
Background Standardized commercially available control material for internal quality control (IQC) of light transmission aggregometry (LTA) is still lacking. Moreover, the availability of normal blood donors to provide fresh platelets is difficult in small laboratories, where 'volunteers' may be in short supply. Objectives To evaluate the implementation of buffy-coat-derived pooled platelet concentrates (PCs) for IQC material for LTA. Methods We used buffy-coat-derived pooled PCs from the blood bank as IQC material for LTA. On each weekend one PC was prepared (> 200 mL) and aliquoted from the original storage bag on a daily basis in four baby bags (40-50 mL), which were delivered from Monday to Friday to our laboratory. The IQC measurements of at least 85 work-weeks (from Monday to Friday) were evaluated with this new IQC material. LTA was performed on a four-channel Chronolog 700 Aggregometer (Chronolog Corporation, Havertown, PA, USA) (agonists: collagen, adenosine diphosphate [ADP], arachidonic acid [AA] and thrombin receptor activator peptide-6 [TRAP-6]). Results The medians of platelet aggregation from IQC measurements with collagen, ADP and AA from Monday to Friday were 68.0-59.5, 3.0-2.0 and 51.0-50.0%, respectively, and the mean of platelet aggregation with TRAP-6 was 71.2-66.4%. Conclusions Buffy-coat-derived pooled PCs serve as a reliable and robust IQC material for LTA measurements and would be beneficial for the whole laboratory procedure and employees' safety.
血小板功能检测中，标准化的内部质控（IQC）目前无法商业化供应。本研究旨在评估使用血库制备的富血小板血浆（PRP）作为光传输聚集检测（LTA）IQC 材料的可行性。
使用血库制备的富血小板血浆作为 IQC 材料进行 LTA。每个周末，从原始储存袋中制备（>200ml）一份富血小板血浆，并在四个婴儿袋中（40-50ml）每天进行分装，从周一到周五送到我们实验室。用这种新的 IQC 材料评估了至少 85 个工作周（周一至周五）的 IQC 测量值。LTA 在 Chronolog 700 四通道聚集仪（Chronolog 公司，哈维敦，PA，美国）上进行（激动剂：胶原、二磷酸腺苷[ADP]、花生四烯酸[AA]和血栓素受体激活肽-6[TRAP-6]）。
周一至周五，IQC 测量中胶原、ADP 和 AA 的血小板聚集中位数分别为 68.0-59.5%、3.0-2.0%和 51.0-50.0%，TRAP-6 的血小板聚集平均值为 71.2-66.4%。
富血小板血浆可作为 LTA 测量的可靠和稳健的 IQC 材料，有利于整个实验室流程和员工安全。
