Raznikov Valeriy V, Zelenov Vladislav V, Aparina Elena V, Pikhtelev Alexander R, Sulimenkov Ilia V, Raznikova Marina O
1 The Branch of Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia.
2 Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia.
Eur J Mass Spectrom (Chichester). 2017 Aug;23(4):181-186. doi: 10.1177/1469066717719466.
The goals of our study were to investigate abilities of two approaches to eliminate possible errors in electrospray mass spectrometry measurements of biomolecules. Passing of a relatively dense supersonic gas jet through ionization region followed by its hitting the spray of the analyzed solution in low vacuum may be effective to keep an initial biomolecule structure in solution. Provided that retention of charge carriers for some sites in the biomolecule cannot be changed noticeably in electrospray ion source, decomposition and separation of charge-state distributions of electrosprayed ions may give additional information about native structure of biomolecules in solution.
我们研究的目的是探究两种方法在消除生物分子电喷雾质谱测量中可能出现的误差方面的能力。让相对致密的超音速气体射流通过电离区域，然后在低真空环境下撞击被分析溶液的喷雾，这可能有效地保持溶液中生物分子的初始结构。假设在电喷雾离子源中生物分子某些位点的电荷载流子保留情况不会发生显著变化，那么电喷雾离子的电荷态分布的分解和分离可能会提供有关溶液中生物分子天然结构的额外信息。
