Waite D J, Sunshine G H
Tufts University Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts.
Cell Immunol. 1988 Nov;117(1):78-88. doi: 10.1016/0008-8749(88)90078-0.
We have investigated the ability of murine T cell lines to induce neonatal tolerance to Mlsa (minor lymphocyte stimulating). Mlsb mice were injected within 24 hr of birth with MHC (major histocompatibility complex) identical T cell lines generated by culturing responders from Mlsa strains with stimulators from Mlsb strains. Injected mice were tested at 6 to 8 weeks of age for responses in either primary mixed leukocyte reaction or IL-2 limiting dilution analysis. Mlsa specific responses by injected tolerant mice relative to noninjected controls were reduced by 92-98% in MLR and by 2- to 10-fold in IL-2 LDA. In contrast, responses against third-party MHC antigens by either the injected or the noninjected mice were identical. Fifty percent of all mice injected with the T cell lines were tolerant to Mlsa. These results strongly suggest that murine T cells express the Mlsa gene product.
我们研究了小鼠T细胞系诱导对Mlsa（次要淋巴细胞刺激）产生新生期耐受性的能力。在出生后24小时内，给Mlsb小鼠注射由用来自Mlsb品系的刺激细胞培养来自Mlsa品系的反应细胞所产生的MHC（主要组织相容性复合体）相同的T细胞系。在6至8周龄时，对注射的小鼠进行原发性混合淋巴细胞反应或IL-2有限稀释分析中的反应测试。相对于未注射的对照，注射的耐受小鼠的Mlsa特异性反应在混合淋巴细胞反应中降低了92 - 98%，在IL-2有限稀释分析中降低了2至10倍。相比之下，注射或未注射的小鼠对第三方MHC抗原的反应是相同的。所有注射T细胞系的小鼠中有50%对Mlsa产生耐受。这些结果强烈表明小鼠T细胞表达Mlsa基因产物。
