Jenski L J, Miller B A, Belloni M L
Department of Biology, Indiana University-Purdue University, Indianapolis 46223.
Immunol Invest. 1987 Aug;16(5):413-23. doi: 10.3109/08820138709087095.
We have determined the frequency of splenic interleukin 2-producing T-cell precursors that respond to H-2-identical, Mls-disparate stimulating cells. Gene products of the Mlsa and Mlsd alleles are highly stimulatory in primary mixed lymphocyte reactions and activate substantial numbers of T-cell precursors in normal spleens. Although the product of the Mlsc allele has been described as weakly stimulatory in primary mixed lymphocyte reactions, we detected abundant T-cell precursors responsive to Mlsc under our limiting dilution conditions; H-2k Mlsa T-cell precursors responding to Mlsc were as frequent (2.605 per 1000) as H-2k Mlsb or Mlsc T-cells responsive to Mlsd (1.975 per 1000). In contrast, using the frequency of self-responding cells as a threshold, we were unable to detect precursors for Mlsa, Mlsb, Mlsc, or Mlsd in H-2b mice, for Mlsb in H-2k mice, or for Mlsc in H-2k Mlsb or Mlsd mice. Thus, we conclude that in select strain combinations and under our experimental conditions Mlsc stimulators can activate T-cell precursors that are as abundant as precursors reactive to strong stimulators Mlsa and Mlsd.
我们已经确定了对H-2相同、Mls不同的刺激细胞产生脾白细胞介素2的T细胞前体的频率。Mlsa和Mlsd等位基因的基因产物在初次混合淋巴细胞反应中具有高度刺激性，并能激活正常脾脏中大量的T细胞前体。尽管Mlsc等位基因的产物在初次混合淋巴细胞反应中被描述为弱刺激性，但在我们的有限稀释条件下，我们检测到大量对Mlsc有反应的T细胞前体；对Mlsc有反应的H-2k Mlsa T细胞前体的频率（每1000个中有2.605个）与对Mlsd有反应的H-2k Mlsb或Mlsc T细胞的频率（每1000个中有1.975个）一样高。相比之下，以自身反应细胞的频率为阈值，我们在H-2b小鼠中未能检测到Mlsa、Mlsb、Mlsc或Mlsd的前体，在H-2k小鼠中未能检测到Mlsb的前体，在H-2k Mlsb或Mlsd小鼠中未能检测到Mlsc的前体。因此，我们得出结论，在特定的品系组合和我们的实验条件下，Mlsc刺激剂可以激活与对强刺激剂Mlsa和Mlsd有反应的前体一样丰富的T细胞前体。
