Department of Oral Biology, School of Dentistry, Wellcome Trust Brenner Building, St. James's University Hospital, University of Leeds, Leeds, LS9 7TF, UK.
Department of Oral Biology, School of Dentistry, Wellcome Trust Brenner Building, St. James's University Hospital, University of Leeds, Leeds, LS9 7TF, UK.
Arch Oral Biol. 2018 Jan;85:46-50. doi: 10.1016/j.archoralbio.2017.09.035. Epub 2017 Oct 3.
The effect of various interventions on enamel demineralisation can be determined by chemically measuring mineral ions dissolved by the attacking acid. Results are usually expressed as mineral loss per surface area of enamel exposed. Acid resistant varnish or adhesive tape are typically used to delineate an area of enamel. However, enamel surface curvature, rugosity and porosity reduce the reliability of simple area measurements made at the macro scale. Our aim was to develop a simple method for investigating the effect of adsorbates on enamel demineralisation that does not rely on knowing the area of enamel exposed. As an exemplar we have used salivary proteins as a model adsorbate.
Natural human tooth enamel surfaces were subjected to five sequential acid challenges and then incubated in adsorbate (whole clarified saliva) followed by a further 15 acid challenges. Demineralisation was determined by measuring the phosphate released into the acid during each exposure by a spectrophotometric assay. The initial five challenges established a mean baseline mineral loss for each tooth against which the effect of subsequently adsorbed proteins could be compared.
Salivary proteins significantly reduced the acid demineralisation of human enamel by 43% (p<0.01). Loss of proteins during each challenge corresponded to a gradual reduction in the degree of protection afforded.
The methodology provides a simple and flexible means to investigate the effect of any adsorbate on enamel acid dissolution. Knowledge of the area of exposed enamel is irrelevant as each tooth acts as its own negative control.
通过化学方法测量被攻击酸溶解的矿物质离子，可以确定各种干预措施对牙釉质脱矿的影响。结果通常表示为暴露于牙釉质表面的每单位面积的矿物质损失。通常使用耐酸漆或胶带来划定牙釉质区域。然而，牙釉质表面的曲率、粗糙度和孔隙率降低了在宏观尺度上进行简单面积测量的可靠性。我们的目的是开发一种简单的方法来研究吸附物对牙釉质脱矿的影响，而无需知道暴露的牙釉质面积。作为一个范例，我们使用唾液蛋白作为模型吸附物。
天然人牙釉质表面经受五次连续的酸挑战，然后在吸附物（全澄清唾液）中孵育，然后再进行 15 次酸挑战。通过分光光度法测定每次暴露过程中释放到酸中的磷酸盐来确定脱矿程度。最初的五次挑战为每个牙齿建立了一个平均基线矿物质损失，随后吸附的蛋白质的效果可以与之进行比较。
唾液蛋白显著降低了人牙釉质的酸脱矿作用，减少了 43%（p<0.01）。每次挑战中蛋白质的损失对应于保护程度的逐渐降低。
该方法为研究任何吸附物对牙釉质酸溶解的影响提供了一种简单灵活的手段。暴露牙釉质面积的知识是无关紧要的，因为每个牙齿都是自己的阴性对照。
