Freake H C, Santos A, Goldberg Y, Ghysdael J, Oppenheimer J H
Department of Medicine, University of Minnesota, Minneapolis 55455.
Mol Endocrinol. 1988 Oct;2(10):986-91. doi: 10.1210/mend-2-10-986.
The in vitro translated products of several c-erbA cDNAs have recently been shown to bind thyroid hormones with high affinity and have been termed thyroid hormone receptors. We have used a panel of five erbA-related antibodies to probe the relationship between c-erbA translated products and thyroid hormone receptors, as conventionally measured by 125I-T3 labeling of nuclear extracts. All five antibodies immunoprecipitated the chick c-erbA translated products, but only one of them recognized chick liver and brain T3 receptor, as judged by acceleration of sedimentation through sucrose gradients. None of the antibodies reacted with rat liver and brain or human liver T3 receptors, although one antibody did immunoprecipitate a human c-erbA translated product. We conclude that the T3 receptor, as conventionally measured from these sources, is related but not identical to recently cloned c-erbA sequences.
最近有研究表明，几种c-erbA cDNA的体外翻译产物能以高亲和力结合甲状腺激素，这些产物被称为甲状腺激素受体。我们使用了一组五种与erbA相关的抗体，来探究c-erbA翻译产物与甲状腺激素受体之间的关系，甲状腺激素受体通常是通过对核提取物进行125I-T3标记来测定的。所有这五种抗体都能免疫沉淀鸡c-erbA翻译产物，但通过蔗糖梯度沉降加速判断，其中只有一种抗体能识别鸡肝和脑的T3受体。尽管有一种抗体确实能免疫沉淀人c-erbA翻译产物，但没有一种抗体能与大鼠肝和脑或人肝的T3受体发生反应。我们得出结论，从这些来源按照常规方法测定的T3受体，与最近克隆的c-erbA序列相关，但并不相同。
