Cui Chen, Huang Ligang, Li Jing, Zou Xingqi, Zhu Yuanyuan, Xie Lei, Zhao Qizu, Yang Limin, Liu Wenjun
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Sheng Wu Gong Cheng Xue Bao. 2016 Nov 25;32(11):1519-1530. doi: 10.13345/j.cjb.160099.
Recombinant structural protein VP1 of foot-and-mouth disease virus serotype O was expressed in Escherichia coli and then purified using Nickel affinity chromatography. A chemiluminescent enzyme immunoassay (CLEIA) method was established using the purified recombinant protein as coating antigen to detect antibody of foot-and-mouth disease virus serotype O in swine. The specificity of VP1-CLEIA method is 100%. The coefficients of variation in the plate and between plates are 1.10%-6.70% and 0.66%-4.80%, respectively. Comparing with the commercial indirect ELISA kit or liquid phase block ELISA kit, the calculated coincidence rate is 93.50% or 94.00%. The high specificity and stability suggested this detection method can be used to monitor the antibody level of foot-and-mouth disease virus serotype O in swine.
口蹄疫病毒O型重组结构蛋白VP1在大肠杆菌中表达，然后通过镍亲和层析进行纯化。以纯化的重组蛋白为包被抗原，建立了一种化学发光酶免疫分析（CLEIA）方法，用于检测猪血清中口蹄疫病毒O型抗体。VP1-CLEIA方法的特异性为100%。板内变异系数和板间变异系数分别为1.10%-6.70%和0.66%-4.80%。与市售间接ELISA试剂盒或液相阻断ELISA试剂盒相比，计算出的符合率分别为93.50%或94.00%。该检测方法具有较高的特异性和稳定性，可用于监测猪口蹄疫病毒O型抗体水平。
