[钙离子在制备交联酶聚集体中的结构调控]
[Structural regulation by calcium ion in preparing cross-linked enzyme aggregates].
作者信息
Han Xiaoqi, Bai Shu, Shi Qinghong
机构信息
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300354, China.
Key Laboratory of System Bioengineering of Ministry of Education, Tianjin University, Tianjin 300354, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2016 Dec 25;32(12):1676-1684. doi: 10.13345/j.cjb.160155.
We studied the effect of calcium ion on particle size and pore structure of cross-linked enzyme aggregates (CLEAs) of glucose oxidase, with activity and stability of the enzyme as evaluation criteria. With calcium ion to prepare CLEA significantly decreased particle sizes of CLEAs whilst the pore structures of CLEAs gradually disappeared with the increase of calcium concentration. When glucose oxidase was precipitated at 0.1 mmol/L Ca²⁺, glucose oxidase in CLEA showed the definitive pore structure. Moreover, glucose oxidase activity in CLEA with Ca²⁺ was 1.69 times higher than that without Ca²⁺. Even at Ca²⁺ as high as 1.0 mmol/L, glucose oxidase activity in CLEA was 42% higher than that of CLEA without Ca²⁺. Furthermore, CLEA prepared with 0.1 mmol/L Ca²⁺ not only exhibited higher substrate conversion and operational stability, but also increased the maximum reaction speed. Therefore, calcium ion improved the performance of glucose oxidase in CLEAs.
我们以葡萄糖氧化酶交联酶聚集体（CLEAs）的活性和稳定性为评价标准，研究了钙离子对其粒径和孔结构的影响。用钙离子制备CLEAs可显著降低其粒径，而随着钙浓度的增加，CLEAs的孔结构逐渐消失。当葡萄糖氧化酶在0.1 mmol/L Ca²⁺ 下沉淀时，CLEA中的葡萄糖氧化酶呈现出明确的孔结构。此外，含Ca²⁺ 的CLEA中葡萄糖氧化酶的活性比不含Ca²⁺ 的高出1.69倍。即使在Ca²⁺ 高达1.0 mmol/L时，含Ca²⁺ 的CLEA中葡萄糖氧化酶的活性仍比不含Ca²⁺ 的CLEA高42%。此外，用0.1 mmol/L Ca²⁺ 制备的CLEA不仅表现出更高的底物转化率和操作稳定性，还提高了最大反应速度。因此，钙离子改善了CLEAs中葡萄糖氧化酶的性能。
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