Department of Chemical Engineering, University of Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
Bioresour Technol. 2011 Jun;102(11):6593-9. doi: 10.1016/j.biortech.2011.03.018. Epub 2011 Mar 14.
Versatile peroxidase (VP) from Bjerkandera adusta was insolubilized in the form of cross-linked enzyme aggregates (CLEA®s). Of the initially applied activity 67% was recovered as CLEA®s. Co-aggregation of VP with glucose oxidase from Aspergillus niger led to an increased activity recovery of 89%. The combined CLEA®s showed higher stability against H(2)O(2) and exerted VP activity upon glucose addition. The elimination of the endocrine disrupting chemicals bisphenol A, nonylphenol, triclosan, 17α-ethinylestradiol and the hormone 17β-estradiol (10 mg L(-1) each) and the removal of their estrogenic activity by combined CLEA®s were tested in batch experiments. Within 10 min, the combined CLEA®s were able to remove all the endocrine disruptors except triclosan (residual concentration 74%). The removal of the estrogenic activity was higher than 55% for all compounds, except triclosan. A membrane reactor continuously operated with combined CLEA®s could almost completely remove bisphenol A (10 mg L(-1)) for 43 h.
杂色云芝过氧化物酶（VP）以交联酶聚集体（CLEA®s）的形式不溶。最初应用的活性中有 67%以 CLEA®s 的形式回收。黑曲霉葡萄糖氧化酶与 VP 的共聚集导致活性回收率增加到 89%。联合 CLEA®s 对 H（2）O（2）表现出更高的稳定性，并在添加葡萄糖时发挥 VP 活性。在批量实验中测试了联合 CLEA®s 对内分泌干扰化学物质双酚 A、壬基酚、三氯生、17α-乙炔雌二醇和激素 17β-雌二醇（每种 10 mg/L）的去除及其雌激素活性的消除。在 10 分钟内，联合 CLEA®s 能够去除除三氯生以外的所有内分泌干扰物（残留浓度 74%）。除三氯生外，所有化合物的雌激素活性去除率均高于 55%。用联合 CLEA®s 连续运行的膜反应器可以在 43 小时内几乎完全去除双酚 A（10 mg/L）。
