Neuman R S, Ben-Ari Y, Gho M, Cherubini E
INSERM U29, Hôpital de Port-Royal, Paris, France.
Neurosci Lett. 1988 Sep 23;92(1):64-8. doi: 10.1016/0304-3940(88)90743-4.
Superfusion of hippocampal slices with 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 2-5 microM) reversibly blocked the Schaffer collateral and mossy fibre excitatory postsynaptic potential (EPSP), while sparing the fast and slow gamma-aminobutyric acid (GABA)-mediated inhibition. Membrane potential, input resistance and spike accommodation were not altered. Inward currents induced by quisqualate were reduced to a greater extent by CNQX than those induced by kainate or N-methyl-D-aspartate. We suggest that CNQX may be a useful antagonist to study excitatory amino acid-mediated synaptic transmission.
用6-氰基-7-硝基喹喔啉-2,3-二酮（CNQX，2 - 5微摩尔）对海马切片进行灌流，可可逆性阻断Schaffer侧支和苔藓纤维兴奋性突触后电位（EPSP），同时保留快速和慢速γ-氨基丁酸（GABA）介导的抑制作用。膜电位、输入电阻和动作电位适应均未改变。CNQX对 quisqualate诱导的内向电流的降低程度大于对 kainate或N-甲基-D-天冬氨酸诱导的内向电流的降低程度。我们认为CNQX可能是研究兴奋性氨基酸介导的突触传递的一种有用拮抗剂。
