Li Xiuqin, Liu Chao, Jiang Bo, Chen Kai, Li Wenjuan, Wang Hongze
Department of Pharmacy, Taizhou People's Hospital, The Fifth Affiliated Hospital of Medical School of Nantong University.
Yangtze River Pharmaceutical Group, Taizhou.
Behav Pharmacol. 2018 Jun;29(4):306-315. doi: 10.1097/FBP.0000000000000350.
Sinomenine is a bioactive alkaloid extracted from Sinomenium acutum. Here, we investigated the antidepressant effects of sinomenine in mice. The antidepressant actions of sinomenine were first examined in the forced-swim test and the tail-suspension test, and then assessed in the chronic social defeat stress (CSDS) model of depression. Changes in the brain-derived neurotrophic factor (BDNF) signaling pathway after CSDS and sinomenine treatment were also investigated. A tryptophan hydroxylase inhibitor and a BDNF signaling inhibitor were also used to determine the pharmacological mechanisms of sinomenine. It was found that sinomenine induced antidepressant-like effects in the forced-swim test and tail-suspension test without affecting the locomotor activity of mice. Sinomenine also prevented the CSDS-induced depressive-like symptoms. Moreover, sinomenine fully restored the CSDS-induced decrease in the hippocampal BDNF signaling pathway, whereas a BDNF signaling inhibitor, but not a tryptophan hydroxylase inhibitor, blocked the antidepressant effects of sinomenine. In conclusion, sinomenine exerts antidepressant effects in mice by promoting the hippocampal BDNF signaling pathway.
青藤碱是从青风藤中提取的一种生物活性生物碱。在此，我们研究了青藤碱对小鼠的抗抑郁作用。首先在强迫游泳试验和悬尾试验中检测青藤碱的抗抑郁作用，然后在慢性社会挫败应激（CSDS）抑郁模型中进行评估。还研究了CSDS和青藤碱治疗后脑源性神经营养因子（BDNF）信号通路的变化。还使用了色氨酸羟化酶抑制剂和BDNF信号抑制剂来确定青藤碱的药理机制。结果发现，青藤碱在强迫游泳试验和悬尾试验中诱导出抗抑郁样作用，而不影响小鼠的运动活性。青藤碱还可预防CSDS诱导的抑郁样症状。此外，青藤碱完全恢复了CSDS诱导的海马BDNF信号通路的降低，而BDNF信号抑制剂而非色氨酸羟化酶抑制剂阻断了青藤碱的抗抑郁作用。总之，青藤碱通过促进海马BDNF信号通路在小鼠中发挥抗抑郁作用。
