Nozari G, Rahbar S, Wallace R B
City of Hope National Medical Center, Duarte, California 91010.
Anal Biochem. 1988 Jul;172(1):180-4. doi: 10.1016/0003-2697(88)90429-0.
Three oligonucleotide probes complementary to specific DNA sequences of the six human globin genes (epsilon, G gamma, A gamma, psi beta, delta, beta) were synthesized. The oligonucleotides were used either singly or in combination as hybridization probes to determine the haplotype of the human beta-globin gene cluster employing the four conventionally used restriction endonucleases HincII, HindIII, AvaII, and BamHI, in addition to HpaI. Polymorphism in the epsilon- and psi beta-genes (HincII) can be simultaneously determined with a single probe mixture. One of the probes complementary to both the psi beta- and gamma-genes is useful for determining both HindIII and HincII polymorphisms. The advantages of these probes relative to conventional cDNA probes are discussed.
合成了与六种人类珠蛋白基因（ε、Gγ、Aγ、ψβ、δ、β）的特定DNA序列互补的三种寡核苷酸探针。这些寡核苷酸单独或组合用作杂交探针，除了HpaI之外，还使用四种常规使用的限制性内切酶HincII、HindIII、AvaII和BamHI来确定人类β-珠蛋白基因簇的单倍型。ε-和ψβ-基因（HincII）中的多态性可以用单一探针混合物同时测定。与ψβ-和γ-基因都互补的一种探针可用于确定HindIII和HincII多态性。讨论了这些探针相对于传统cDNA探针的优势。
