School of Chemical Engineering, The University of New South Wales, Sydney, New South Wales 2052, Australia.
Marine Bioproducts Technology, Kyoto University, Kyoto 606-8502, Japan.
Food Chem. 2018 Mar 1;242:247-255. doi: 10.1016/j.foodchem.2017.09.075. Epub 2017 Sep 15.
The chemical and biological properties of carotenoids in the freshwater alga Oedogonium intermedium were investigated in this study. Carotenoids were extracted from the alga by dichloromethane and purified by saponification. The carotenoid content was determined both spectrometrically and by HPLC, the carotenoids identified by HPLC-PDA-APCI-IT-TOF-MS and the extracts analysed for several health-related bioactivities. The crude and saponified extracts contained 3,411.2±20.7 and 2,929.6±5.9µg carotenoids/g dry algal biomass, respectively. Seven major carotenoids were identified, namely neoxanthin, 9'-cis-neoxanthin, loroxanthin, violaxanthin, lutein, α-carotene and β-carotene, which were present in similar amounts in the alga. Both the crude and saponified carotenoid extracts exhibited significant antioxidant activities as well as potent inhibitory effects against several metabolically important enzymes including α-amylase, α-glucosidase, pancreatic lipase and hyaluronidase, but they were poor inhibitors of angiotensin converting enzyme (ACE). Oedogonium could be an important new source of carotenoids, specifically loroxanthin, which is lacking in terrestrial plants.
本研究旨在探究淡水藻类中交链藻（Oedogonium intermedium）中的类胡萝卜素的化学和生物学特性。通过二氯甲烷从藻类中提取类胡萝卜素，并通过皂化进行纯化。通过光谱法和 HPLC 确定类胡萝卜素的含量，通过 HPLC-PDA-APCI-IT-TOF-MS 鉴定类胡萝卜素，通过提取物分析几种与健康相关的生物活性。粗提取物和皂化提取物分别含有 3,411.2±20.7 和 2,929.6±5.9µg 类胡萝卜素/g 干藻生物质。鉴定出七种主要的类胡萝卜素，即新黄质、9'-顺式新黄质、洛罗黄质、叶黄素、α-胡萝卜素、β-胡萝卜素，它们在藻类中的含量相似。粗提物和皂化提取物均表现出显著的抗氧化活性，以及对几种代谢重要酶（包括α-淀粉酶、α-葡萄糖苷酶、胰脂肪酶和透明质酸酶）的有效抑制作用，但对血管紧张素转换酶（ACE）的抑制作用较弱。交链藻可能是类胡萝卜素的一个重要新来源，特别是洛罗黄质，这在陆生植物中是缺乏的。
