Guchhait Rajkumar, Chatterjee Ankit, Gupta Shreyasi, Debnath Manashi, Mukherjee Dilip, Pramanick Kousik
Integrative Biology Research Unit, Department of Life Sciences, Presidency University, 86/1, College Street, Kolkata 700073, India.
Department of Zoology, University of Kalyani, Kalyani 741235, India.
Gen Comp Endocrinol. 2018 Jan 1;255:40-48. doi: 10.1016/j.ygcen.2017.10.004. Epub 2017 Oct 14.
Mercury is one of the key pollutants responsible for the degradation of natural aquatic ecosystems. Among the different forms of mercury that exist in the environment, mercuric chloride (HgCl) is the dominant pollutant for freshwater environments as it is used as an ingredient in antiseptics, disinfectants and preservatives, insecticides, batteries and in metallurgical and photographic operations. Pollutant may exert their action on organisms or populations by affecting their normal endocrine function as well as reproduction. Thus, the present study tried to understand the effect of mercuric chloride (HgCl) on reproductive function and to decipher the molecular mechanism of Hg-induced reproductive impairments of female Trichogaster fasciata. Both in vivo and in vitro experiments were performed by using ecologically relevant doses of HgCl and the resulting effects on follicular development, steroidogenic potentiality, aromatase activity, aromatase gene expression and steroidogenic factor-1 (SF-1) expression pattern were analysed. In vivo exposure to HgCl caused reproductive impairments as shown by the inhibitory role of HgCl on follicular development, steroid biosynthesis and SF-1 activity. In vitro experiments revealed that aromatase activity, steroidogenesis, aromatase and SF-1 expression were blocked by HgCl. The results obtained from this study contribute to understand the molecular mechanism of HgCl-induced reproductive impairment of T. fasciata.
汞是导致自然水生生态系统退化的关键污染物之一。在环境中存在的不同形式的汞中，氯化汞（HgCl）是淡水环境中的主要污染物，因为它被用作防腐剂、消毒剂和防腐剂、杀虫剂、电池以及冶金和摄影操作中的一种成分。污染物可能通过影响生物体或种群的正常内分泌功能以及繁殖来发挥其作用。因此，本研究试图了解氯化汞（HgCl）对生殖功能的影响，并破译汞诱导的雌性条纹毛足鲈生殖损伤的分子机制。通过使用与生态相关剂量的HgCl进行体内和体外实验，并分析其对卵泡发育、类固醇生成潜力、芳香化酶活性、芳香化酶基因表达和类固醇生成因子-1（SF-1）表达模式的影响。体内暴露于HgCl会导致生殖损伤，如HgCl对卵泡发育、类固醇生物合成和SF-1活性的抑制作用所示。体外实验表明，HgCl会阻断芳香化酶活性、类固醇生成、芳香化酶和SF-1的表达。本研究获得的结果有助于了解HgCl诱导条纹毛足鲈生殖损伤的分子机制。
