Mochida Keiichi, Shinozaki Kazuo
Cellulose Production Research Team, RIKEN Center for Sustainable Resource Science, #E804 8F East Research Bldg., 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama, Kanagawa, 244-0813, Japan.
Methods Mol Biol. 2018;1667:87-99. doi: 10.1007/978-1-4939-7278-4_8.
Online tools and databases play an essential role in the promotion of functional genomics studies. Several resources for information regarding Brachypodium distachyon (Brachypodium) are available on the Web. In this chapter, we focus on recently published resources for Brachypodium research. The Brachypodium.org website ( http://www.brachypodium.org /) is an information portal that provides links to various genomic resources regarding Brachypodium, including genome annotation and re-sequencing datasets of accessions. RIKEN Full-length cDNA Database (RBFLDB, http://brachy.bmep.riken.jp/ver.1/index.pl ) is a web-accessible database that provides information of Brachypodium full-length cDNAs (FLcDNAs) collected in RIKEN and updated gene structures of Brachypodium based on the FLcDNA sequences as well as results of comparative analyses with available sequence resources for Triticeae crops, wheat, and barley. We introduce the functionalities and availability of these important information resources. Furthermore, we also present brief descriptions of useful online tools that facilitate Brachypodium functional genomics studies.
在线工具和数据库在促进功能基因组学研究方面发挥着重要作用。网络上有一些关于二穗短柄草(Brachypodium)信息的资源。在本章中,我们重点关注最近发布的二穗短柄草研究资源。Brachypodium.org网站(http://www.brachypodium.org/)是一个信息门户,提供指向各种关于二穗短柄草基因组资源的链接,包括基因组注释和种质的重测序数据集。日本理化研究所全长cDNA数据库(RBFLDB,http://brachy.bmep.riken.jp/ver.1/index.pl)是一个可通过网络访问的数据库,它提供了在日本理化研究所收集的二穗短柄草全长cDNA(FLcDNA)信息,以及基于FLcDNA序列更新的二穗短柄草基因结构,还有与小麦和大麦等小麦族作物现有序列资源的比较分析结果。我们介绍这些重要信息资源的功能和可用性。此外,我们还简要描述了有助于二穗短柄草功能基因组学研究的有用在线工具。