功能转基因方法：短柄草高效转化方案的开发及其对推进短柄草转基因研究的适用性

Methods for Functional Transgenics: Development of Highly Efficient Transformation Protocol in Brachypodium and Its Suitability for Advancing Brachypodium Transgenics.

作者信息

Vunsh Ron

机构信息

Department of Plant and Environmental Sciences, The Weizmann Institute of Science, 234 Herzl Street, Rehovot, Israel.

出版信息

Methods Mol Biol. 2018;1667:101-117. doi: 10.1007/978-1-4939-7278-4_9.

DOI:10.1007/978-1-4939-7278-4_9

PMID:29039007

Abstract

Plant transformation is an invaluable technique in plant genomics by which an extra foreign DNA sequence is introduced into a plant genome. Changing the plant genome is leading to owning new genetic characteristics. Model plant is a keystone in a study of the comprehensive plant phylum. Here, I describe an efficient method to transform the plant species Brachypodium distachyon which, due to its characters, is developing to be an important plant model.

摘要

植物转化是植物基因组学中一项极具价值的技术，通过该技术可将额外的外源DNA序列导入植物基因组。改变植物基因组会使其具备新的遗传特性。模式植物是整个植物门类研究中的关键要素。在此，我描述一种转化植物物种短柄草的高效方法，由于其自身特性，短柄草正逐渐成为一种重要的植物模式。