达什穆拉瑞什塔口服多草药制剂对骨关节炎小鼠模型的评估。
Evaluation of oral multi-herbal preparation of Dashmoolarishta on mice model of osteoarthritis.
作者信息
Shetty Yashashri C, Godbharle Santosh, Brahma Smita, Salgaonkar Sweta, Rege Nirmala N
机构信息
.
出版信息
J Basic Clin Physiol Pharmacol. 2017 Nov 27;28(6):583-591. doi: 10.1515/jbcpp-2016-0141.
BACKGROUND
Osteoarthritis (OA) is a chronic progressive disease commonly affecting the hip and knee joints. Although synthetic drugs are available and afford symptomatic relief, their side effects pose limitations to their continuous use. So, this research was focused on extracting drugs from indigenous medicinal plants that could have a beneficial effect on osteoarthritis. Dashmoolarishta is one such preparation whose effects have never been studied in comparison with recent drugs like hyaluronic acid (HA), hence this particular study was undertaken. The aim of this study was to evaluate the effects of Dashmoolarishta compared with HA on joint pathology and pain behavior in monosodiumiodoacetate (MIA)-induced OA in experimental mice.
METHODS
The study was initiated after obtaining permission from the Animal Ethics Committee. This study was based on the MIA model of osteoarthritis, with mice being divided into five groups viz.: disease control (DC), Dasahmoolarishta high dose (HD) and low dose (LD), sham control (SC) and HA. The OA of the knee joint was induced in these mice using monosodiumiodoacetate. Seven days after induction, animals were subjected to weekly behavioral tests, daily oral Dashmoolarishta, and biweekly HA administration from weeks 2-4. At the end of the 4th week, histopathological examination of the knee joints was done.
RESULTS
DC showed significant osteoarthritic changes. At week 4, the behavioral tests and histopathology results of all groups were found to be significant. A significant difference (p<0.05) was found between DC vs. SC, HA, HD, LD for open field test, Rota rod test, knee joint diameter, and Cat walk test. Dashmoolarishta HD and LD showed significant improvement in pain, as assessed by behavioral tests (p<0.05) and pathology, as assessed by knee joint histopathology (p<0.05).
CONCLUSIONS
Oral Dashmoolarishta showed reduction in pain and disease activity in MIA-induced osteoarthritis in mice model.
背景
骨关节炎（OA）是一种常见的慢性进行性疾病，通常影响髋关节和膝关节。尽管有合成药物可用于缓解症状，但其副作用限制了它们的持续使用。因此，本研究聚焦于从本土药用植物中提取可能对骨关节炎有有益作用的药物。达什穆拉里斯塔就是这样一种制剂，其效果从未与透明质酸（HA）等近期药物进行过比较，因此开展了这项特定研究。本研究的目的是评估达什穆拉里斯塔与HA相比，对实验性小鼠单碘乙酸钠（MIA）诱导的骨关节炎关节病理和疼痛行为的影响。
方法
在获得动物伦理委员会许可后启动本研究。本研究基于骨关节炎的MIA模型，将小鼠分为五组，即：疾病对照组（DC）、达什穆拉里斯塔高剂量组（HD）和低剂量组（LD）、假手术对照组（SC）和HA组。使用单碘乙酸钠在这些小鼠中诱导膝关节骨关节炎。诱导7天后，动物每周接受行为测试，从第2周开始每天口服达什穆拉里斯塔，从第2 - 4周每两周给予一次HA。在第4周结束时，对膝关节进行组织病理学检查。
结果
DC组显示出明显的骨关节炎变化。在第4周时，发现所有组的行为测试和组织病理学结果均有显著差异。在旷场试验、转棒试验、膝关节直径和猫步试验中，DC组与SC组、HA组、HD组、LD组之间存在显著差异（p<0.05）。通过行为测试评估（p<0.05）以及通过膝关节组织病理学评估（p<0.05），达什穆拉里斯塔HD组和LD组在疼痛方面显示出显著改善。
结论
在小鼠模型中，口服达什穆拉里斯塔可减轻MIA诱导的骨关节炎的疼痛和疾病活动。
Zotero 插件