School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Biosens Bioelectron. 2018 Mar 15;101:52-59. doi: 10.1016/j.bios.2017.10.003. Epub 2017 Oct 7.
In this work, we demonstrated a nano-decorated porous impedance electrode sensor for efficient capture, rapid killing and ultrasensitive detection of bacteria. The multi-functional sensor was prepared by a facile sonochemical method via in situ deposition of antibacterial prickly Zn-CuO nanoparticles and graphene oxide (GO) nanosheets on a Ni porous electrode. Due to the surface burr-like nanostructures, the nano-decorated impedance sensor exhibited very good bacterial-capture efficiency (70 - 80% in 20min) even at a low concentration of 50 CFU mL, rapid antibacterial rate (100% killing in 30min) and high detection sensitivity (as low as 10 CFU mL). More importantly, the nano-decorated sensor has proven to be highly effective in quantitative detection of bacteria in a biological sample, for example, a rat blood sample spiked with E. coli. Despite the complexity of blood, the sensor still exhibited excellent detection precision within 30min at bacteria concentrations ranging from 10 - 10 CFU mL. The simplicity, rapidity, sensitivity, practicability and multifunctionality of this impedance sensor would greatly facilitate applications in portable medical devices for on-the-spot diagnosis and even the possibility for simultaneous therapy of diseases caused by bacterial infections.
在这项工作中，我们展示了一种纳米修饰的多孔阻抗电极传感器，用于高效捕获、快速杀灭和超灵敏检测细菌。多功能传感器是通过简便的超声化学方法制备的，通过在 Ni 多孔电极上原位沉积抗菌刺状 Zn-CuO 纳米粒子和氧化石墨烯（GO）纳米片来实现。由于表面呈毛刺状的纳米结构，纳米修饰的阻抗传感器即使在低浓度为 50 CFU mL 的情况下也表现出非常好的细菌捕获效率（20min 内为 70-80%），快速杀菌率（30min 内 100%杀灭）和高检测灵敏度（低至 10 CFU mL）。更重要的是，纳米修饰的传感器已被证明在生物样品中定量检测细菌非常有效，例如，在大肠杆菌污染的大鼠血液样本中。尽管血液复杂，但该传感器在 30min 内仍能在 10-10 CFU mL 的细菌浓度范围内表现出出色的检测精度。这种阻抗传感器的简单性、快速性、灵敏度、实用性和多功能性将极大地方便用于便携式医疗设备中进行现场诊断，甚至有可能同时治疗由细菌感染引起的疾病。
