Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru,560030, India.
Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru,560030, India.
Biomed Pharmacother. 2017 Dec;96:685-694. doi: 10.1016/j.biopha.2017.09.151. Epub 2017 Nov 6.
Arsenic, often referred to as the king of poisons is carcinogenic in humans and animals. It affects multiorgan systems including reproduction. The present study was undertaken to explore the protective role of green tea compound, epigallocatechin-3-gallate (EGCG) on arsenic induced testicular toxicity in Swiss albino mice. Thirty two adult male mice were randomly assigned to four groups (n=8). Group I served as control without test chemical. The group II received arsenic (200ppm) through drinking water, group III received only EGCG (20mg/kgb.wt., intraperitoneally, alternate days) and group IV was administered arsenic+EGCG for 40days. Factorial experimental design was employed to assess the treatment effect. The EGCG restored arsenic induced decrements in epididymal sperm concentration, kinematic attributes (total motility, rapid, progressive motile, fast progressive, VSL, VAP, VCL, BCF, LIN, WOB, STR and Type A), structutal membrane integrity, functional membrane integrity and mitochondrial membrane potential. As evidenced by the histoarchitectural studies, the EGCG reversed the deleterious effects of arsenic on testicular malondialdehyde (p<0.05) levels, reduced glutathione, antioxidative enzymes and spermatogenesis. Overall, the results suggest that EGCG reduces the testicular oxidative stress induced by arsenic poisoning and thereby protect the reproductive system.
砷，通常被称为“毒中之王”，在人类和动物中具有致癌性。它影响包括生殖系统在内的多个器官系统。本研究旨在探索绿茶化合物表没食子儿茶素没食子酸酯（EGCG）对瑞士白化雄性小鼠砷诱导睾丸毒性的保护作用。32 只成年雄性小鼠被随机分为四组（n=8）。第 I 组作为对照组，不给予受试化学物质。第 II 组通过饮用水摄入砷（200ppm），第 III 组仅给予 EGCG（20mg/kgb.wt.，腹腔内，隔日一次），第 IV 组给予砷+EGCG 共 40 天。采用析因实验设计来评估治疗效果。EGCG 恢复了砷诱导的附睾精子浓度、运动学特性（总活力、快速、渐进运动、快速渐进运动、VSL、VAP、VCL、BCF、LIN、WOB、STR 和 A 型）、结构膜完整性、功能膜完整性和线粒体膜电位的降低。如组织形态学研究所示，EGCG 逆转了砷对睾丸丙二醛（p<0.05）水平、还原型谷胱甘肽、抗氧化酶和精子发生的有害影响。总的来说，结果表明 EGCG 可减轻砷中毒引起的睾丸氧化应激，从而保护生殖系统。
