使用固定有适配体的原子力显微镜芯片检测丙型肝炎病毒核心抗原。
The detection of hepatitis c virus core antigen using afm chips with immobolized aptamers.
作者信息
Pleshakova T O, Kaysheva A L, Bayzyanova J М, Anashkina А S, Uchaikin V F, Ziborov V S, Konev V A, Archakov A I, Ivanov Y D
机构信息
Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121, Russia.
Institute of Biomedical Chemistry, Pogodinskaya St. 10, Moscow, 119121, Russia.
出版信息
J Virol Methods. 2018 Jan;251:99-105. doi: 10.1016/j.jviromet.2017.10.015. Epub 2017 Oct 16.
In the present study, the possibility of hepatitis C virus core antigen (HCVcoreAg) detection in buffer solution, using atomic force microscope chip (AFM-chip) with immobilized aptamers, has been demonstrated. The target protein was detected in 1mL of solution at concentrations from 10М to 10М. The registration of aptamer/antigen complexes on the chip surface was carried out by atomic force microscopy (AFM). The further mass-spectrometric (MS) identification of AFM-registered objects on the chip surface allowed reliable identification of HCVcoreAg target protein in the complexes. Aptamers, which were designed for therapeutic purposes, have been shown to be effective in HCVcoreAg detection as probe molecules.
在本研究中，已证明使用固定有适配体的原子力显微镜芯片（AFM芯片）在缓冲溶液中检测丙型肝炎病毒核心抗原（HCVcoreAg）的可能性。在1mL浓度范围为10М至10М的溶液中检测到了目标蛋白。通过原子力显微镜（AFM）对芯片表面的适配体/抗原复合物进行记录。对芯片表面AFM记录的物体进行进一步的质谱（MS）鉴定，从而可靠地鉴定出复合物中的HCVcoreAg目标蛋白。已表明，设计用于治疗目的的适配体作为探针分子在HCVcoreAg检测中是有效的。
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