Lou Beibei, Liu Yanfei, Shi Meilin, Chen Jun, Li Ke, Tan Yifu, Chen Liwei, Wu Yuwei, Wang Ting, Liu Xiaoqin, Jiang Ting, Peng Dongming, Liu Zhenbao
Department of Pharmaceutics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, Hunan Province, PR China.
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, Hunan Province, PR China.
Trends Analyt Chem. 2022 Dec;157:116738. doi: 10.1016/j.trac.2022.116738. Epub 2022 Jul 19.
Virus threatens life health seriously. The accurate early diagnosis of the virus is vital for clinical control and treatment of virus infection. Aptamers are small single-stranded oligonucleotides (DNAs or RNAs). In this review, we summarized aptasensors for virus detection in recent years according to the classification of the viral target protein, and illustrated common detection mechanisms in the aptasensors (colorimetry, fluorescence assay, surface plasmon resonance (SPR), surface-enhanced raman spectroscopy (SERS), electrochemical detection, and field-effect transistor (FET)). Furthermore, aptamers against different target proteins of viruses were summarized. The relationships between the different biomarkers of the viruses and the detection methods, and their performances were revealed. In addition, the challenges and future directions of aptasensors were discussed. This review will provide valuable references for constructing on-site aptasensors for detecting viruses, especially the SARS-CoV-2.
病毒严重威胁生命健康。病毒的准确早期诊断对于临床控制和治疗病毒感染至关重要。适配体是小的单链寡核苷酸（DNA或RNA）。在本综述中，我们根据病毒靶蛋白的分类总结了近年来用于病毒检测的适配体传感器，并阐述了适配体传感器中的常见检测机制（比色法、荧光测定法、表面等离子体共振（SPR）、表面增强拉曼光谱（SERS）、电化学检测和场效应晶体管（FET））。此外，还总结了针对病毒不同靶蛋白的适配体。揭示了病毒不同生物标志物与检测方法之间的关系及其性能。此外，还讨论了适配体传感器面临的挑战和未来方向。本综述将为构建用于检测病毒，尤其是严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的现场适配体传感器提供有价值的参考。
