Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 210009, China.
College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
Int J Biol Macromol. 2018 Feb;107(Pt B):2150-2156. doi: 10.1016/j.ijbiomac.2017.10.090. Epub 2017 Oct 16.
A polysaccharide (FVSP) was isolated from the base of Flammuliana Velutipes stipe, and FVSP was further purified by DEAE-cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to obtain three fractions named FVSP-1, FVSP-2 and FVSP-3. Then their activation of macrophage cell RAW 264.7 and anti-proliferative effects to the murine melanoma B16F10 and fibroblasts L929 cells were evaluated by using the cell model experiments. The results indicated that the polysaccharide fractions could increase the proliferation and phagocytic activity of macrophage significantly and play an inhibited effect on the cancer cells. Moreover, the anti-proliferative activities of FVSPs increased with the participation of the antitumor factors induced from macrophage by polysaccharides fractions. Taken together, these results indicated that three polysaccharides fractions from the base of F. Velutipes stipe may be useful as potent antitumor agents for the prevention of tumorigenesis.
一种多糖（FVSP）从金顶侧耳菌菌柄底部分离得到，并用 DEAE-纤维素-52 层析和 Sephadex G-100 排阻层析进一步纯化 FVSP，得到三个级分，命名为 FVSP-1、FVSP-2 和 FVSP-3。然后通过细胞模型实验评估了它们对巨噬细胞 RAW 264.7 的激活作用和对鼠黑色素瘤 B16F10 和成纤维细胞 L929 细胞的抗增殖作用。结果表明，多糖级分可显著增加巨噬细胞的增殖和吞噬活性，并对癌细胞发挥抑制作用。此外，FVSPs 的抗增殖活性随着多糖级分诱导的巨噬细胞中抗肿瘤因子的参与而增加。总之，这些结果表明，金顶侧耳菌菌柄底部的三种多糖级分可能可用作预防肿瘤发生的有效抗肿瘤剂。
Zotero 插件