Han Yu, Hao Huili, Yang Lihong, Chen Guolian, Wen Yucong, Huang Riming
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University Guangzhou 510642 China
Shenzhen Shajing People's Hospital, Guangzhou University of Chinese Medicine Shenzhen China
RSC Adv. 2019 Sep 24;9(52):30144-30153. doi: 10.1039/c9ra04913d. eCollection 2019 Sep 23.
Bleeker, an edible and medicinal marine fish, is largely distributed in tropical oceans. However, the chemical composition and nutritional properties of this species have not yet been investigated. In the present study, proximate composition, fatty acids, amino acids, taurine, and minerals of Bleeker were characterized, and the immunostimulatory properties of its glycoprotein were evaluated. The results indicated the presence of crude protein (19.66%), crude lipid (6.29%) and carbohydrate (0.74%) in Bleeker; monounsaturated fatty acids and polyunsaturated fatty acids in the fatty acid composition of Bleeker were 25.00% and 31.01%, respectively; Bleeker was rich in taurine (219 mg/100 g) and essential amino acids (5.57 g/100 g). In addition, the glycoprotein of consisted of protein and sugars, with a total content of 34.25% and 16.27%, respectively. The glycoprotein showed significant effects on promoting NO, TNF-α and IL-6 in a dose-dependent manner in RAW264.7 macrophage cells. Thus, these findings provide a scientific basis for the further utilization of glycoprotein from Bleeker.
布氏鲳鲹是一种可食用的药用海鱼,广泛分布于热带海洋。然而,该物种的化学成分和营养特性尚未得到研究。在本研究中,对布氏鲳鲹的近似成分、脂肪酸、氨基酸、牛磺酸和矿物质进行了表征,并评估了其糖蛋白的免疫刺激特性。结果表明,布氏鲳鲹中含有粗蛋白(19.66%)、粗脂肪(6.29%)和碳水化合物(0.74%);布氏鲳鲹脂肪酸组成中的单不饱和脂肪酸和多不饱和脂肪酸分别为25.00%和31.01%;布氏鲳鲹富含牛磺酸(219毫克/100克)和必需氨基酸(5.57克/100克)。此外,布氏鲳鲹糖蛋白由蛋白质和糖类组成,其总含量分别为34.25%和16.27%。该糖蛋白在RAW264.7巨噬细胞中对促进一氧化氮、肿瘤坏死因子-α和白细胞介素-6呈剂量依赖性显著作用。因此,这些发现为布氏鲳鲹糖蛋白的进一步利用提供了科学依据。