内质网（ER）膜脂质组成与代谢：脂质组学分析

ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis.

作者信息

Fouillen Laetitia, Maneta-Peyret Lilly, Moreau Patrick

机构信息

CNRS-University of Bordeaux, UMR 5200 Membrane Biogenesis Laboratory, INRA Bordeaux Aquitaine, 33140, Villenave d'Ornon, France.

MetaboHub-Metabolome Facility of Bordeaux, Functional Genomics Center, Bordeaux, France.

出版信息

Methods Mol Biol. 2018;1691:125-137. doi: 10.1007/978-1-4939-7389-7_10.

DOI:10.1007/978-1-4939-7389-7_10

PMID:29043674

Abstract

Plant ER membranes are the major site of biosynthesis of several lipid families (phospholipids, sphingolipids, neutral lipids such as sterols and triacylglycerols). The structural diversity of lipids presents considerable challenges to comprehensive lipid analysis. This chapter will briefly review the various biosynthetic pathways and will detail several aspects of the lipid analysis: lipid extraction, handling, separation, detection, identification, and data presentation. The different tools/approaches used for lipid analysis will also be discussed in relation to the studies to be carried out on lipid metabolism and function.

摘要

植物内质网膜是几种脂质家族（磷脂、鞘脂、中性脂质如甾醇和三酰甘油）生物合成的主要场所。脂质的结构多样性给全面的脂质分析带来了相当大的挑战。本章将简要回顾各种生物合成途径，并详细介绍脂质分析的几个方面：脂质提取、处理、分离、检测、鉴定和数据呈现。还将讨论用于脂质分析的不同工具/方法与脂质代谢和功能研究的相关性。