金纳米颗粒对DNA修饰具有高稳定性，并通过无表面活性剂的肽途径实现高效杂交。
High stability of gold nanoparticles towards DNA modification and efficient hybridization via a surfactant-free peptide route.
作者信息
Li Xin-Yi, Feng Fu-Yan, Wu Zi-Tong, Liu Yi-Zhen, Zhou Xiao-Dong, Hu Ji-Ming
机构信息
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
出版信息
Chem Commun (Camb). 2017 Oct 31;53(87):11909-11912. doi: 10.1039/c7cc06827a.
With the assistance of peptides, a stable and highly efficient approach for the quick loading of thiol-DNA onto AuNPs is proposed with a high DNA utilization, which is generally applicable to specific DNA detection and diverse AuNP sizes. The maximum efficiency of hybridization reached 93% while the reaction time was shortened to 30 min.
在肽的辅助下，提出了一种稳定且高效的方法，可将硫醇化DNA快速加载到金纳米颗粒上，DNA利用率高，该方法普遍适用于特定DNA检测和不同尺寸的金纳米颗粒。杂交效率最高可达93%，同时反应时间缩短至30分钟。
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