Turner C E, Newton M R, Shotton D M
Department of Zoology, University of Oxford, England.
J Cell Sci. 1988 Mar;89 ( Pt 3):309-19. doi: 10.1242/jcs.89.3.309.
The independent capping of the three major rat thymocyte glycoproteins, the leucocyte-common (L-C) antigen, the leucocyte sialoglycoprotein (LSGP) and Thy-1, was investigated using specific monoclonal antibodies. The capping of each antigen did not require redistribution of the other major surface glycoproteins, and was accompanied by a partial co-capping of the cytoskeletal proteins fodrin and actin, but not of tubulin. A study of the ability of a cell that already possesses one glycoprotein cap to cap a second different glycoprotein showed that this was possible in all cases to varying degrees, the second cap always forming at the same position on the cell surface as the first. Colchicine failed to perturb this observed sequential capping polarity, indicating that microtubules did not direct this second capping event.
利用特异性单克隆抗体对大鼠三种主要胸腺细胞糖蛋白（白细胞共同抗原（L-C）、白细胞唾液糖蛋白（LSGP）和Thy-1）的独立帽化进行了研究。每种抗原的帽化并不需要其他主要表面糖蛋白的重新分布，并且伴随着细胞骨架蛋白血影蛋白和肌动蛋白的部分共帽化，但微管蛋白没有。对已经具有一个糖蛋白帽的细胞帽化第二种不同糖蛋白的能力进行的研究表明，在所有情况下这在不同程度上都是可能的，第二个帽总是在细胞表面与第一个帽相同的位置形成。秋水仙碱未能干扰观察到的这种顺序帽化极性，表明微管并未指导这第二次帽化事件。
