Hashimoto Ai, Karim Mohammad Rabiul, Izawa Takeshi, Kuwamura Mitsuru, Yamate Jyoji
Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ourai-Kita, Izumisano-shi, Osaka 598-8531, Japan.
J Vet Med Sci. 2017 Dec 6;79(12):1920-1926. doi: 10.1292/jvms.17-0423. Epub 2017 Oct 17.
Pancreatic fibrosis develops as the results of the activity of myofibroblasts capable of producing collagens. The myofibroblasts derive from pancreatic interstitial cells, including pancreatic stellate cells (PSCs), which can express glial fibrillary acidic protein (GFAP). First, we investigated the expression patterns of vimentin, desmin, α-smooth muscle actin (α-SMA), Thy-1 and GFAP in the developing rat pancreas (in fetuses at 18 and 20 days, neonates from 1 to 21 days, and adults). Interstitial cells in the developing pancreas expressed vimentin, desmin, GFAP and Thy-1 at varying degrees; interestingly, the reactivity for desmin and vimentin was the highest in fetuses. GFAP expression was consistent between fetuses and neonates, and Thy-1 reactivity transiently increased after birth; however, α-SMA-positive interstitial cells were rarely seen. Next, we analyzed the immunophenotypical characteristics of myofibroblasts appearing in pancreatic fibrosis in dogs and cats. With increasing fibrotic grade, myofibroblasts showed increased expression of vimentin, desmin and α-SMA, in addition to increased GFAP expression. Collectively, pancreatic interstitial cells and myofibroblasts may have similar immunophenotypes, and myofibroblasts might originate partly from GFAP-expressing PSCs.
胰腺纤维化是由能够产生胶原蛋白的肌成纤维细胞的活动导致的。肌成纤维细胞来源于胰腺间质细胞,包括胰腺星状细胞(PSCs),后者可表达胶质纤维酸性蛋白(GFAP)。首先,我们研究了波形蛋白、结蛋白、α平滑肌肌动蛋白(α-SMA)、Thy-1和GFAP在发育中的大鼠胰腺(18和20天的胎儿、1至21天的新生儿以及成年大鼠)中的表达模式。发育中的胰腺间质细胞不同程度地表达波形蛋白、结蛋白、GFAP和Thy-1;有趣的是,结蛋白和波形蛋白的反应性在胎儿中最高。胎儿和新生儿的GFAP表达一致,Thy-1反应性在出生后短暂增加;然而,很少见到α-SMA阳性间质细胞。接下来,我们分析了犬猫胰腺纤维化中出现的肌成纤维细胞的免疫表型特征。随着纤维化程度的增加,肌成纤维细胞除了GFAP表达增加外,波形蛋白、结蛋白和α-SMA的表达也增加。总体而言,胰腺间质细胞和肌成纤维细胞可能具有相似的免疫表型,并且肌成纤维细胞可能部分起源于表达GFAP的PSCs。