Pontual Emmanuel V, Pires-Neto Divar F, Fraige Karina, Higino Taciana M M, Carvalho Belany E A, Alves Neyla M P, Lima Thâmarah A, Zingali Russolina B, Coelho Luana C B B, Bolzani Vanderlan S, Figueiredo Regina C B Q, Napoleão Thiago H, Paiva Patrícia M G
a Departamento de Morfologia e Fisiologia Animal , Universidade Federal Rural de Pernambuco , Recife , Brazil.
b Departamento de Microbiologia , Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz , Recife , Brazil.
Nat Prod Res. 2018 Dec;32(24):2940-2944. doi: 10.1080/14786419.2017.1389932. Epub 2017 Oct 19.
In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC of 54.18 ± 6.62 and 41.20 ± 4.28 μg/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract.
在本研究中，对辣木花提取物及其从中分离出的一种胰蛋白酶抑制剂（MoFTI）进行了针对克氏锥虫的抗原虫活性以及对哺乳动物细胞的细胞毒性评估。在提取物的高效液相色谱指纹图谱中，254和360nm处黄酮类化合物的存在显著。测定了从MoFTI凝胶内消化衍生的肽的氨基酸序列。提取物和MoFTI均导致克氏锥虫无鞭毛体裂解，LC分别为54.18±6.62和41.20±4.28μg/mL。提取物和MoFTI对克氏锥虫细胞相对于小鼠腹腔巨噬细胞和Vero细胞具有高选择性指数（7.9至>12）。结果表明，MoFTI是花提取物的杀锥虫成分。
