Verspohl Alexandra, Pignedoli Samuele, Giudici Paolo
University of Modena and Reggio Emilia, Department of Life Sciences, Via Amedola 2, 42122, Reggio Emilia, Italy.
Yeast. 2018 Jan;35(1):173-187. doi: 10.1002/yea.3288. Epub 2017 Dec 12.
The inheritance of mitochondrial DNA (mtDNA) in yeast hybrids is heteroplasmic, turning into homoplasmic after few cell cycles. Mitotype restoration is reported to be biparental and random. This study shows that mitotype restoration follows fixed schemata. We created isogenic, interspecific Saccharomyces cerevisiae × Saccharomyces uvarum hybrids through direct mating and analysed their mating frequency and mitotype. The mating frequency increased for most crosses in staggered mating. Mitotyping revealed that breeding lines with the same parental strain of S. cerevisiae and different parental strains of S. uvarum give rise to the same mitotype. According to our results, we postulate that the inheritance of mtDNA is dominated by one parental strain and that the superior mitotype is specific to each breeding line. Technological tests showed typical hybrid heterosis. Copyright © 2017 John Wiley & Sons, Ltd.
酵母杂交体中线粒体DNA（mtDNA）的遗传是异质性的，经过几个细胞周期后会转变为同质性。据报道，线粒体类型的恢复是双亲本且随机的。本研究表明，线粒体类型的恢复遵循固定模式。我们通过直接交配创建了同基因的种间酿酒酵母×葡萄汁酵母杂交体，并分析了它们的交配频率和线粒体类型。在交错交配中，大多数杂交组合的交配频率增加。线粒体分型显示，具有相同酿酒酵母亲本菌株和不同葡萄汁酵母亲本菌株的育种系产生相同的线粒体类型。根据我们的结果，我们推测mtDNA的遗传由一个亲本菌株主导，并且优势线粒体类型对每个育种系都是特定的。技术测试显示出典型的杂种优势。版权所有© 2017约翰威立父子有限公司。
