Wang Hongwei, Liu Zhongshan, Peng Xiaojun, Ou Junjie, Ye Mingliang
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
Se Pu. 2017 Jul 8;35(7):688-695. doi: 10.3724/SP.J.1123.2017.05014.
An acylsemicarbazide-based porous organic polymer (POP) was facilely prepared by the polymerization of benzene-1,3,5-tricarbohydrazide (BTZ) and 1,4-phenylene diisocyanate (PDI). The physical properties of as-synthesized material were characterized by Fourier-transform infrared spectroscopy (FT-IR), solid-state cross polarisation magic angle spinning carbon-13 nuclear magnetic resonance (CP-MAS C NMR), nitrogen adsorption/desorption measurement, water contact angle and so on. The specific surface area was 166 m/g, and the water contact angle was 46.4°, exhibiting hydrophilic property of the porous material. Thus, POP-1 was tried to be used for glycopeptides enrichment from tryptic digest of standard protein and complex biosamples in hydrophilic mode. The 19 typical -linked glycopeptides were identified from 500 fmol tryptic digest of immunoglobulin G (IgG) from human serum by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis, meanwhile, 1919 unique glycopeptides with 1350 -glycosylation sites from 605 different -linked glycoproteins were identified from 100 μ g mouse liver tryptic digest by capillary liquid chromatography (cLC)-MS/MS analysis. These results demonstrated the great potential of POP-1 for glycoproteome analysis.
通过苯-1,3,5-三碳酰肼（BTZ）和1,4-亚苯基二异氰酸酯（PDI）聚合，简便地制备了一种基于酰基氨基脲的多孔有机聚合物（POP）。采用傅里叶变换红外光谱（FT-IR）、固态交叉极化魔角旋转碳-13核磁共振（CP-MAS C NMR）、氮气吸附/脱附测量、水接触角等手段对合成材料的物理性质进行了表征。其比表面积为166 m²/g，水接触角为46.4°，表明该多孔材料具有亲水性。因此，尝试将POP-1用于在亲水模式下从标准蛋白质的胰蛋白酶消化物和复杂生物样品中富集糖肽。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）分析，从人血清免疫球蛋白G（IgG）的500 fmol胰蛋白酶消化物中鉴定出19种典型的N-连接糖肽，同时，通过毛细管液相色谱（cLC）-MS/MS分析，从100μg小鼠肝脏胰蛋白酶消化物中鉴定出1919种来自605种不同N-连接糖蛋白的具有1350个N-糖基化位点的独特糖肽。这些结果证明了POP-1在糖蛋白质组分析方面具有巨大潜力。
