Graziano S L, Mark G E, Murray C, Mann D L, Ehrlich G D, Poiesz B J, Weston A
Department of Medicine, SUNY-Health Science Center, Syracuse 13210.
Oncogene Res. 1988;3(1):99-103.
We report the identification and characterization of two restriction fragment polymorphisms (RFLPs) associated with the human c-raf-1 protooncogene located on chromosome 3 (3p25). EcoRI digestion produces an RFLP derived from the 5'flanking sequences with a heterozygosity of 28%, and TaqI produces an RFLP representing the 3' flanking sequences with a heterozygosity of 25%. The finding of RFLPs associated with a potential oncogene may provide a method of analysis for determining genetic susceptibilities to certain cancers.
我们报告了与位于3号染色体（3p25）上的人类c-raf-1原癌基因相关的两种限制性片段长度多态性（RFLP）的鉴定与特征分析。用EcoRI酶切产生一种源自5'侧翼序列的RFLP，杂合度为28%，而用TaqI酶切产生一种代表3'侧翼序列的RFLP，杂合度为25%。与潜在癌基因相关的RFLP的发现可能为确定某些癌症的遗传易感性提供一种分析方法。
