Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, University of Tehran, Karaj 31587-77871, Iran.
Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, University of Tehran, Karaj 31587-77871, Iran.
Carbohydr Polym. 2017 Dec 15;178:27-33. doi: 10.1016/j.carbpol.2017.08.098. Epub 2017 Aug 24.
In this study, the effect of aqueous extraction conditions (temperature of 70-90°C, time of 60-180min, and liquid/solid ratio (LSR) of 20-40v/w) on the yield and degree of esterification (DE) of Citrus medica peel pectin was studied using a Box-Behnken design. The highest production yield of pectin (21.85±0.35%) was obtained at temperature of 90°C, extraction time of 180min and LSR of 40v/w as optimum extraction conditions which was close to the predicted value (24.13%). In these extraction conditions, the DE and the emulsifying activity were 77.2 and 46.5%, respectively. Also, the emulsions were 90.30 and 90% stable at 4°C, and 83.87 and 83.50% at 25°C after 1 and 30days, respectively. The determination of flow behavior showed that the pectin solutions had a Newtonian behavior at low concentrations (0.1, 0.5 and 1.0%w/v), while in higher concentration (2.0%w/v), the pseudoplastic flow behavior became dominant.
在这项研究中，使用 Box-Behnken 设计研究了水提取条件（温度为 70-90°C、时间为 60-180min 和液固比（LSR）为 20-40v/w）对柚皮果胶产率和酯化度（DE）的影响。在最佳提取条件下（温度为 90°C、提取时间为 180min 和 LSR 为 40v/w），果胶的最高产量（21.85±0.35%）接近预测值（24.13%）。在这些提取条件下，DE 和乳化活性分别为 77.2%和 46.5%。此外，在 4°C 下，乳液在 1 天和 30 天后分别稳定在 90.30%和 90%，在 25°C 下分别稳定在 83.87%和 83.50%。流变性的测定表明，果胶溶液在低浓度（0.1、0.5 和 1.0%w/v）下表现出牛顿行为，而在较高浓度（2.0%w/v）下，假塑性流动行为占主导地位。
