从 2D 到 3D：丝素蛋白/壳聚糖基质上 MC3T3-E1 的形态、增殖和分化。
From 2D to 3D: The morphology, proliferation and differentiation of MC3T3-E1 on silk fibroin/chitosan matrices.
机构信息
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China.
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China.
出版信息
Carbohydr Polym. 2017 Dec 15;178:69-77. doi: 10.1016/j.carbpol.2017.09.035. Epub 2017 Sep 12.
It has been widely accepted that cell culture in two-dimensional (2D) conditions may not be able to represent growth in three-dimensional (3D) conditions. Systematic comparisons between 2D and 3D cell cultures are needed to appropriately use the existing 2D results. In this work, we conducted a comparative study between 2D and 3D cell cultures of MC3T3-E1 using the same type of material (a mixture of silk fibroin (SF) and chitosan (CS)). Our results showed 3D SF/CS scaffold exhibited different effects on cell culture compared with the 2D cases. 1) The cells grown in 3D scaffold showed multiple morphologies. 2) The proliferation of cells in 3D scaffold was long-term and sustainable. 3) Cell differentiation occurred throughout the entire 3D scaffold. The results showed that cell culture in 3D SF/CS scaffold exhibited different features than 2D cases and 3D SF/CS scaffold could be a promising material for 3D cell culture.
人们普遍认为，二维（2D）条件下的细胞培养可能无法代表三维（3D）条件下的生长。需要对 2D 和 3D 细胞培养进行系统比较，以便正确使用现有的 2D 结果。在这项工作中，我们使用相同类型的材料（丝素蛋白（SF）和壳聚糖（CS）的混合物）对 MC3T3-E1 的 2D 和 3D 细胞培养进行了比较研究。我们的结果表明，3D SF/CS 支架对细胞培养的影响与 2D 情况不同。1）在 3D 支架中生长的细胞呈现出多种形态。2）3D 支架中细胞的增殖是长期的和可持续的。3）细胞分化发生在整个 3D 支架中。结果表明，3D SF/CS 支架中的细胞培养表现出与 2D 情况不同的特征，3D SF/CS 支架可能是 3D 细胞培养的一种有前途的材料。
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