a Department of Soil Science , Islamic Azad University, Science and Research Branch , Tehran , Iran.
b Soil and Water Research Institute , Karaj , Iran.
Int J Phytoremediation. 2018 Apr 16;20(5):476-482. doi: 10.1080/15226514.2017.1374330.
Oil presence in soil, as a stressor, reduces phytoremediation efficiency through an increase in the plant stress ethylene. Bacterial 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, as a plant stress ethylene reducer, was employed to increase oil phytoremediation efficiency. For this purpose, the ability of ACC deaminase-producing Pseudomonas strains to grow in oil-polluted culture media and withstand various concentrations of oil and also their ability to reduce plant stress ethylene and enhance some growth characteristics of maize and finally their effects on increasing phytoremediation efficiency of poly aromatic hydrocarbons (PAHs) in soil were investigated. Based on the results, of tested strains just P9 and P12 were able to perform oil degradation. Increasing oil concentration from 0 to 10% augmented these two strains population, 15.7% and 12.9%, respectively. The maximum increase in maize growth was observed in presence of P12 strain. Results of high-performance liquid chromatography (HPLC) revealed that PAHs phytoremediation efficiency was higher for inoculated seeds than uninoculated. The highest plant growth and PAHs removal percentage (74.9%) from oil-polluted soil was observed in maize inoculated with P12. These results indicate the significance of ACC deaminase producing bacteria in alleviation of plant stress ethylene in oil-polluted soils and increasing phytoremediation efficiency of such soils.
油类物质作为一种胁迫源存在于土壤中，会通过增加植物胁迫乙烯来降低植物修复效率。细菌 1-氨基环丙烷-1-羧酸（ACC）脱氨酶作为一种植物胁迫乙烯的还原剂，被用于提高油类的植物修复效率。为此，研究了产 ACC 脱氨酶的假单胞菌菌株在受油污染的培养基中生长的能力，以及它们耐受各种浓度油的能力，还研究了它们降低植物胁迫乙烯和增强玉米某些生长特性的能力，最后研究了它们对提高土壤中多环芳烃（PAHs）植物修复效率的影响。结果表明，在所测试的菌株中，只有 P9 和 P12 能够进行油类降解。将油浓度从 0 增加到 10%，分别使这两个菌株的数量增加了 15.7%和 12.9%。在 P12 菌株存在的情况下，玉米的生长增加幅度最大。高效液相色谱（HPLC）的结果表明，接种种子的多环芳烃植物修复效率高于未接种的种子。在接种 P12 的玉米中，从受油污染的土壤中去除植物生长和 PAHs 的比例最高（74.9%）。这些结果表明，产 ACC 脱氨酶的细菌在缓解油污染土壤中植物胁迫乙烯和提高此类土壤的植物修复效率方面具有重要意义。
