Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, PR China; Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, Shanghai 200444, PR China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China.
Anal Chim Acta. 2017 Nov 1;992:85-93. doi: 10.1016/j.aca.2017.09.002. Epub 2017 Sep 13.
A new strategy is proposed based on inhibition of ion transport by lipid bilayer derived from spontaneous assembly of lipopolysaccharides (LPS), thereby a colorimetric method is established for analysis of LPS. At acidic pH values, LPS can specially bind with aminophenylboronic acid modified assembled magnetic nanospheres (APBA/AMNSs), resulting in formation of lipid bilayer around APBA/AMNSs. Under acidic condition, the lipid bilayer can inhibit the release of iron ions from AMNSs into the solution so as to decrease the oxidized extent of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt mediated by hydrogen peroxide. Using the established method, LPS can be detected over the wide linear detection range with the low detection limit. With good selectivity, reproductivity, and simplicity, the method is accurate in LPS tests of real drinking samples.
基于脂多糖（LPS）自发组装衍生的脂质双层对离子传输的抑制作用，提出了一种新策略，从而建立了一种用于分析 LPS 的比色法。在酸性 pH 值下，LPS 可以特异性地与氨苯基硼酸修饰的组装磁纳米球（APBA/AMNSs）结合，导致 APBA/AMNSs 周围形成脂质双层。在酸性条件下，脂质双层可以抑制铁离子从 AMNSs 释放到溶液中，从而减少由过氧化物介导的 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）二铵盐的氧化程度。利用所建立的方法，可以在宽线性检测范围内以低检测限检测 LPS。该方法具有良好的选择性、重现性和简单性，在实际饮用样品的 LPS 测试中准确可靠。
