Huan Beili, Liu Ke, Li Yuming, Wei Jianchao, Shao Donghua, Shi Yuanyuan, Qiu Yafeng, Li Beibei, Ma Zhiyong
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai, 200241, PR China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai, 200241, PR China; Key Laboratory for Veterinary Chemical Drugs and Pharmaceutics, Ministry of Agriculture of China, No. 518, Ziyue Road, Shanghai, 200241, PR China.
Dev Comp Immunol. 2018 Feb;79:51-58. doi: 10.1016/j.dci.2017.10.007. Epub 2017 Oct 19.
Serum amyloid A (SAA) is an acute phase protein that is expressed rapidly in response to infection and inflammation in vertebrates. Here, we detected the expression of porcine SAA3, an isoform of porcine SAA, during porcine respiratory and reproductive syndrome virus (PRRSV) infection, which is a major threat to the pig industry. In response to PRRSV infection, porcine SAA3 expression was upregulated significantly in porcine pulmonary alveolar macrophages and in extrahepatic tissues, including the lungs and inguinal, mandibular, and hilar lymph nodes, which were affected mainly by PRRSV infection, demonstrating that porcine SAA3 is a tissue-derived isoform that is induced in extrahepatic tissues during the acute phase response. Overexpression of porcine SAA3 increased PRRSV titers in cultured cells, and the exogenous administration of porcine SAA3 facilitated PRRSV adsorption to cells, suggesting that porcine SAA3 assists PRRSV replication. Our data provide insights into the role of porcine SAA3 during PRRSV infection.
血清淀粉样蛋白A（SAA）是一种急性期蛋白，在脊椎动物受到感染和炎症刺激时会迅速表达。在此，我们检测了猪SAA的一种同工型——猪SAA3在猪繁殖与呼吸综合征病毒（PRRSV）感染期间的表达情况，PRRSV感染是养猪业面临的主要威胁。在PRRSV感染后，猪肺泡巨噬细胞以及包括肺、腹股沟、下颌和肺门淋巴结在内的肝外组织中，猪SAA3的表达显著上调，这些组织主要受到PRRSV感染的影响，这表明猪SAA3是一种组织衍生的同工型，在急性期反应期间在肝外组织中被诱导产生。猪SAA3的过表达增加了培养细胞中的PRRSV滴度，并且外源性给予猪SAA3促进了PRRSV对细胞的吸附，这表明猪SAA3有助于PRRSV复制。我们的数据为猪SAA3在PRRSV感染过程中的作用提供了见解。
