镉处理后条纹鲈（Morone saxatilis）中金属结合蛋白的诱导。

Induction of metal binding proteins in striped bass, Morone saxatilis, following cadmium treatment.

Baksi S M, Libbus N, Frazier J M

Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;91(2):355-63. doi: 10.1016/0742-8413(88)90042-4.

Abstract

The time course of induction of cytosolic metal binding proteins (MBP) was observed follow up to three daily intramuscular injections of cadmium chloride (0.2 mg cadmium/injection). 2. Low molecular weight binding proteins were resolved by gel permeation chromatography on Sephadex G-75. Based on total metal binding capacity, the concentration of crude MBP increased 2.6 fold. This level of induction of MBP was confirmed by polarographic analysis. 3. Initial binding of cadmium to MBP resulted in displacement of zinc, while at later times, zinc associated with MBP increased above control levels. 4. Using 35S-cysteine incorporation, it was shown that the rate of hepatic MBP synthesis was significantly greater than controls and sham injected fish 18 hr after the third cadmium injection. 5. Due to interfering proteins of molecular weights similar to the metal binding proteins one dimensional PAGE was not capable of verifying induction. However, the metal binding proteins were resolved using two dimensional gel electrophoresis.

摘要

在连续三天每日进行肌肉注射氯化镉（每注射0.2毫克镉）后，对胞质金属结合蛋白（MBP）的诱导时间进程进行了观察。2. 通过在Sephadex G - 75上进行凝胶渗透色谱法分离低分子量结合蛋白。基于总金属结合能力，粗制MBP的浓度增加了2.6倍。MBP的这种诱导水平通过极谱分析得到证实。3. 镉与MBP的初始结合导致锌的置换，而在后期，与MBP结合的锌增加至高于对照水平。4. 使用35S - 半胱氨酸掺入法表明，在第三次注射镉18小时后，肝脏MBP的合成速率显著高于对照组和假注射鱼。5. 由于存在分子量与金属结合蛋白相似的干扰蛋白，一维PAGE无法验证诱导情况。然而，使用二维凝胶电泳分离出了金属结合蛋白。