遗传性近交系小鼠中组成型及艾氏剂1254诱导的肝脏谷胱甘肽S-转移酶、过氧化物酶和还原酶活性

Constitutive and Aroclor 1254-induced hepatic glutathione S-transferase, peroxidase and reductase activities in genetically inbred mice.

Makary M, Kim H L, Safe S, Womack J, Ivie G W

Veterinary Physiology and Pharmacology and Pathology, Texas A&M University, College Station 77843.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;91(2):425-9. doi: 10.1016/0742-8413(88)90054-0.

Abstract

Constitutive and Aroclor 1254-induced hepatic glutathione (GSH) S-transferases, GSH peroxidase and GSH reductase activities were determined in 12 strains of 8-10 week-old inbred male mice. 2. The constitutive GSH S-transferase activity varied from 2.5 (SJL/JCR) to 8.9 (C57BL/6N) mumol/min/mg protein and the corresponding values for the Aroclor 1254-treated mice were in the range of 7.1-23.0 mumol/min/mg protein. Aroclor 1254 significantly induced GSH S-transferase activity in all mice, however, significant interstrain differences were found in inducibility. 3. Aroclor 1254-treatment caused a 4.2-fold induction of GSH S-transferase in NFS/NCR but only a 1.4-fold increase in AKR/NCR mice. Aroclor 1254 significantly induced GSH reductase in all strains studied while GSH peroxidase activity decreased in these mice. 4. The range of hepatic GSH levels in control and Aroclor 1254-treated mice was relatively narrow for both groups (6.59-11.25 microM/g wet tissue).

摘要

测定了12株8 - 10周龄近交系雄性小鼠体内组成型及艾氏剂1254诱导的肝脏谷胱甘肽（GSH）S - 转移酶、GSH过氧化物酶和GSH还原酶的活性。2. 组成型GSH S - 转移酶活性在2.5（SJL/JCR）至8.9（C57BL/6N）μmol/min/mg蛋白之间变化，经艾氏剂1254处理的小鼠相应活性值在7.1 - 23.0μmol/min/mg蛋白范围内。艾氏剂1254在所有小鼠中均显著诱导了GSH S - 转移酶活性，然而，在诱导性方面发现了显著的品系间差异。3. 艾氏剂1254处理使NFS/NCR小鼠的GSH S - 转移酶诱导了4.2倍，但在AKR/NCR小鼠中仅增加了1.4倍。艾氏剂1254在所有研究品系中均显著诱导了GSH还原酶，而这些小鼠的GSH过氧化物酶活性降低。4. 对照组和经艾氏剂1254处理的小鼠肝脏GSH水平范围对两组而言都相对较窄（6.59 - 11.25μM/g湿组织）。