暴露于煤液中的虹鳟（Salmo gairdneri）肝脏芳烃羟化酶活性的诱导。

Induction of hepatic aryl hydrocarbon hydroxylase activity in rainbow trout (Salmo gairdneri) exposed to coal liquids.

Dauble D D, Dankovic D A, Abernethy C S, Gray R H

Pacific Northwest Laboratory, Richland, Washington, D.C. 99352.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;91(2):431-5. doi: 10.1016/0742-8413(88)90055-2.

Abstract

The hepatotoxic response of rainbow trout (Salmo gairdneri) to a middle distillate (MD) and heavy distillate (HD) coal liquid was determined following administration by injection or in food. Hepatic aryl hydrocarbon hydroxylase (AHH) activity was compared to the known AHH inducer, benzo(a)pyrene. 2. Acute toxicities (LD50) of 1500 and 1410 mg/kg were obtained 120 hr after intraperitoneal injection (i.p.) for MD and HD coal liquids, respectively. Only the HD induced AHH activity when injected at 5% and 10% of the acute LD50. 3. Ingestion of 7 or 70 mg HD/kg body weight for 1 week resulted in AHH induction at 5-7 times that of control values. Levels of hepatic AHH activity in fish treated at 7 mg HD/kg declined to 2.6 times control values 7 days after exposure. Maximum induction was observed for the 70 mg HD/kg group that was provided control food for 1 week following 2 weeks of exposure. 4. The hepatic index (HI) or liver to body weight ratio generally increased along with increased activity in the enzyme response system.

摘要

通过注射或添加到食物中给药后，测定虹鳟（Salmo gairdneri）对中间馏分油（MD）和重馏分油（HD）煤液的肝毒性反应。将肝脏芳烃羟化酶（AHH）活性与已知的AHH诱导剂苯并(a)芘进行比较。2. 腹腔注射（i.p.）后120小时，MD和HD煤液的急性毒性（LD50）分别为1500和1410毫克/千克。仅当以急性LD50的5%和10%注射时，HD才诱导AHH活性。3. 以7或70毫克HD/千克体重的剂量摄入1周会导致AHH诱导，诱导水平是对照值的5至7倍。以7毫克HD/千克处理的鱼，其肝脏AHH活性水平在暴露7天后降至对照值的2.6倍。在暴露2周后为70毫克HD/千克组提供1周对照食物，观察到最大诱导效果。4. 肝脏指数（HI）或肝重与体重之比通常随着酶反应系统活性的增加而增加。