Ritt Michelle G, Lindborg Beth A, O'Brien Timothy D, Bisignano Joseph, Modiano Jaime F
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Vet Sci. 2015 Apr 10;2(2):43-51. doi: 10.3390/vetsci2020043.
The characteristics of canine IL-17-producing cells are incompletely understood. Expression of mRNA encoding orthologs of IL-17 and the IL-17 receptor has been documented in tissues from dogs with arthritis, inflammatory bowel disease, and lymphoma; however, no associations have been found between IL-17 gene expression and disease phenotype in these conditions. Robust assessment of the role of IL-17-producing cells in dogs will require measuring the frequency of these cells in health and disease in balance with other lymphocyte subsets. The aim of this study was to confirm that the T-cell IL-17 response in dogs is evolutionarily conserved. Canine peripheral blood mononuclear cells were stimulated with Concanavalin A with or without polarizing cytokines. We used a canine specific IL-17 ELISA and flow cytometry to identify IL-17-producing T cells. Accumulation of intracellular IL-17 was observed in stimulated CD4 and CD8 T cells. The addition of pro-inflammatory cytokines appeared to enhance polarization of canine CD4 T cells to the Th17 phenotype. Conversely, the addition of IL-2 in the presence of TGF-β resulted in expansion of Treg cells. We conclude that canine IL-17-producing cells behave similarly to those from humans and mice when stimulated with mitogens and polarized with pro-inflammatory or immune regulatory cytokines.
犬类产生白细胞介素-17（IL-17）的细胞的特征尚未完全明确。在患有关节炎、炎症性肠病和淋巴瘤的犬类组织中，已记录到编码IL-17及其受体直系同源物的mRNA的表达；然而，在这些病症中，尚未发现IL-17基因表达与疾病表型之间存在关联。要对犬类中产生IL-17的细胞的作用进行有力评估，需要在健康和疾病状态下，结合其他淋巴细胞亚群来测量这些细胞的频率。本研究的目的是证实犬类T细胞的IL-17反应在进化上是保守的。用伴刀豆球蛋白A（Concanavalin A）刺激犬类外周血单个核细胞，同时添加或不添加极化细胞因子。我们使用犬类特异性IL-17酶联免疫吸附测定（ELISA）和流式细胞术来鉴定产生IL-17的T细胞。在受刺激的CD4和CD8 T细胞中观察到细胞内IL-17的积累。添加促炎细胞因子似乎增强了犬类CD4 T细胞向Th17表型的极化。相反，在转化生长因子-β（TGF-β）存在的情况下添加白细胞介素-2（IL-2）导致调节性T细胞（Treg细胞）的扩增。我们得出结论，当用丝裂原刺激并用促炎或免疫调节细胞因子极化时，犬类产生IL-17的细胞的行为与人类和小鼠的细胞相似。
