Schmitz Silke, Garden Oliver A, Werling Dirk, Allenspach Karin
Royal Veterinary College, University of London, Department of Veterinary Clinical Sciences, Hawkshead Campus, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK.
Vet Immunol Immunopathol. 2012 Mar 15;146(1):87-91. doi: 10.1016/j.vetimm.2012.01.013. Epub 2012 Jan 21.
Inflammatory bowel disease (IBD) is a common cause of chronic diarrhoea in dogs. In people, specific cytokine patterns attributed to T cell subsets, especially T helper cell [Th]1, Th17 and regulatory T(reg) cells have emerged in IBD. In contrast, no specific involvement of a distinct T cell subset has been described so far in canine IBD. Thus, the aim of the present study was to assess gene expression of signature cytokines in duodenal tissues from 18 German shepherd dogs with IBD (group 1), 33 dogs of other breeds with IBD (group 2) and 15 control dogs (group 3). Relative quantification of IL-17A, IL-22, IL-10, IFNy and TGFβ was performed. Expression of IL-17A was significantly lower in groups 1 and 2 compared to group 3 (p=0.014), but no difference in the expression of IL-22 (p=0.839), IFNγ (p=0.359), IL-10 (p=0.085) or TGFβ (p=0.551) across groups was detected. Thus, no clear evidence for the involvement of Th-17 signature cytokines in canine IBD at the mRNA level could be shown. The contribution of specific T cell subsets to the pathogenesis of canine IBD warrants further investigation.
炎症性肠病（IBD）是犬慢性腹泻的常见病因。在人类中，IBD已出现了归因于T细胞亚群的特定细胞因子模式，尤其是辅助性T细胞[Th]1、Th17和调节性T（reg）细胞。相比之下，迄今为止在犬IBD中尚未发现有特定的T细胞亚群参与。因此，本研究的目的是评估18只患有IBD的德国牧羊犬（第1组）、33只患有IBD的其他品种犬（第2组）和15只对照犬（第3组）十二指肠组织中标志性细胞因子的基因表达。对IL-17A、IL-22、IL-10、IFNγ和TGFβ进行了相对定量。与第3组相比，第1组和第2组中IL-17A的表达显著降低（p=0.014），但未检测到各组间IL-22（p=0.839）、IFNγ（p=0.359）、IL-10（p=0.085）或TGFβ（p=0.551）表达的差异。因此，在mRNA水平上，没有明确证据表明Th-17标志性细胞因子参与犬IBD。特定T细胞亚群对犬IBD发病机制的作用值得进一步研究。
