Cockcroft Peter D, Redfern Helen E
School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA 5371, Australia.
Glenthorne Veterinary Centre, Uttoxeter, Staffordshire ST148EB, UK.
Vet Sci. 2015 Aug 14;2(3):206-209. doi: 10.3390/vetsci2030206.
The aim of this study was to compare the bacterial load of unclipped gluteal skin in dairy cows following either no treatment or treatment with a standard 70% isopropyl alcohol-based skin treatment protocol. Twenty Holstein-Friesian dairy cows from a commercial dairy herd in Cambridgeshire, England, were used in this randomised, blinded, controlled study. On each of the experimental cows an area of unclipped gluteal skin on one side of the pelvis was treated with swabs soaked in 70% isopropyl alcohol-based using a standard protocol and a contra-lateral area of skin was left untreated as a control. All the experimental skin sites were sampled using a swab followed by bacterial culture and quantitative analysis of bacterial load. There was a statistically significant decrease in the bacterial colony forming units per mL for the isopropyl-alcohol treatment group when compared to the control group ( ≤ 0.01). There was a 58% reduction in the median bacterial load of the treated sites when compared to the bacterial load of the untreated sites. This study has demonstrated that the treatment protocol will reduce the skin bacterial load.
本研究的目的是比较未修剪臀部分皮肤的奶牛在不进行任何处理或采用基于70%异丙醇的标准皮肤处理方案处理后的细菌载量。本随机、双盲、对照研究使用了来自英国剑桥郡一个商业奶牛场的20头荷斯坦-弗里生奶牛。在每头实验奶牛的骨盆一侧，用浸有70%异丙醇的拭子按照标准方案处理一块未修剪的臀部分皮肤区域，对侧皮肤区域作为对照不进行处理。所有实验皮肤部位均用拭子采样，随后进行细菌培养和细菌载量的定量分析。与对照组相比，异丙醇处理组每毫升细菌菌落形成单位有统计学显著下降（≤0.01）。与未处理部位的细菌载量相比，处理部位的细菌载量中位数降低了58%。本研究表明，该处理方案将降低皮肤细菌载量。
