Lewis S J, Verberne A J, Summers R J, Beart P M, Cincotta M
University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Austin Hospital, Heidelberg, Victoria, Australia.
Brain Res Bull. 1988 Dec;21(6):913-6. doi: 10.1016/0361-9230(88)90026-3.
Quantitative receptor autoradiography with L-[3H]glutamate was employed to examine the distribution and properties of glutamate binding sites in the rat brain 14 days after excision of the right nodose ganglion. Slide-mounted coronal sections of the brain showed reduced L-[3H]glutamate binding in the nucleus tractus solitarius/dorsal motor nucleus of the vagus in the ipsilateral relative to the sham-operated side. Densitometric and saturation analyses of binding data indicated a significant reduction in the density of glutamate binding sites (57% decrease relative to sham), while there was a significant increase in receptor affinity (40% greater than sham). Binding was unaltered in the inferior olivary complex. Glutamate receptors are likely to exist on synaptic nerve terminals of vagal afferent fibres within the nucleus tractus solitarius and on vagal preganglionic neurones within the dorsal motor nucleus of the vagus and/or their dendritic processes within the nucleus tractus solitarius. Additionally, our receptor autoradiographic studies provide evidence for L-glutamate being a transmitter of vagal afferent neurones.
采用L-[3H]谷氨酸进行定量受体放射自显影，以检查大鼠右侧结状神经节切除14天后大脑中谷氨酸结合位点的分布和特性。脑冠状切片显示，与假手术侧相比，同侧孤束核/迷走神经背运动核中L-[3H]谷氨酸结合减少。结合数据的光密度分析和饱和分析表明，谷氨酸结合位点密度显著降低（相对于假手术组降低57%），而受体亲和力显著增加（比假手术组高40%）。下橄榄复合体中的结合未改变。谷氨酸受体可能存在于孤束核内迷走神经传入纤维的突触神经末梢、迷走神经背运动核内的迷走神经节前神经元和/或孤束核内它们的树突过程上。此外，我们的受体放射自显影研究为L-谷氨酸是迷走神经传入神经元的递质提供了证据。
