Yang Yi-Ning, Wang Xiao-Rui, Qin You-Wen, Wan Li-Ping, Jiang Ying, Wang Chun
Department of Hematology, Shanghai Jiao Tong University affiliated with Shanghai General Hospital, Shanghai 200000, China.
Chronic Dis Transl Med. 2015 Mar 23;1(1):48-54. doi: 10.1016/j.cdtm.2015.02.004. eCollection 2015 Mar.
To determine the sensitivity and significance of B-cell chimerism for the detection of early engraftment, transplant rejection, and disease relapse.
The dynamic monitoring of lineage-specific cell subtypes (B, T, and NK cells) was made in 20 B-cell acute lymphoblastic leukemia (B-ALL) patients following allogeneic hematopoietic stem cell transplantation (allo-HSCT). In the early period after allo-HSCT, the latest establishment of B-cell complete chimerism (CC) was observed in a majority of patients.
The percentage of donor cells of B-cell lineage was lower than the percent of T-cell lineage in most of the mixed chimerism (MC) patients. During graft rejection, the frequency of patients with decreasing MC of B-, T- and NK-cell lineage were 5/5, 2/5, and 2/5. When disease relapsed, five patients showed a faster decrease of the donor percent of B-cells than of T- or NK-cells. Only one patient displayed a more rapid decrease in NK-cells than in T- or B-cells.
Monitoring of B-cell chimerism after HSCT seems to be valuable for insuring complete engraftment, anticipating graft rejection, and relapse in B-ALL patients.
