抑肽酶纳米体形式的设计。

Design of Nanosomal Form of Aprotinin.

作者信息

Kulikov O A, Pyataev N A, Zaborovskii A V, Maev I V, Ageev V P, Marochkina E E, Dolgacheva I S, Avdyushkina I S, Kokorev A V, Gurevich K G, Tatarina L A, Yunina D V

机构信息

N. P. Ogarev Mordovia National Research State University, Saransk, the Republic of Mordovia, Russia.

A. I. Evdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of Russia, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2017 Oct;163(6):742-744. doi: 10.1007/s10517-017-3893-9. Epub 2017 Oct 24.

DOI:10.1007/s10517-017-3893-9

PMID:29063327

Abstract

Liposomes containing aprotinin were produced by the phase inversion technique and purified by gel filtration. Aprotinin inclusion was assessed fluorescence labeling of the protein. The size of obtained liposomes was 212±35 nm and aprotinin concentration in the liposomal suspension was 3000 KIU/ml. The efficiency of aprotinin inclusion into liposomes was 31.9%.

摘要

通过相转化技术制备含抑肽酶的脂质体，并通过凝胶过滤进行纯化。采用蛋白质荧光标记法评估抑肽酶的包封情况。所制备脂质体的大小为212±35 nm，脂质体悬浮液中抑肽酶的浓度为3000 KIU/ml。抑肽酶包封入脂质体的效率为31.9%。