Yan Da-Zhong, Gan Ya-Ting, Zhou Hui, Liu Jun, Li Xin
School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, No.68 Xuefu South Road, Changqing Garden, Wuhan, 430023, Hubei Province, China.
Curr Microbiol. 2018 Mar;75(3):284-287. doi: 10.1007/s00284-017-1377-9. Epub 2017 Oct 24.
Acinetobacter sp. YT-02, a Gram-negative bacterium isolated from the activated sludge from a sodium N-cyclohexylsulfamate production plant, has the ability to degrade cyclohexylamine. It was classified as a member of Acinetobacter sp., a Gram-negative bacterium, sharing a 16S rRNA gene sequence identity of 99% with Acinetobacter guangdongensis strain 1NM-4. It could degrade 10 mmol/L cyclohexylamine within 22 h. Based on the identified metabolite, the metabolic pathway of cyclohexylamine could be postulated as it was degraded via cyclohexanone. Draft genome sequence of this strain (2,993, 647 bp of chromosome length) is presented here. We further identified the genes encoding the enzymes involved in cyclohexylamine oxidation to cyclohexanone and the subsequent downstream metabolic pathway of cyclohexanone oxidation. Strain YT-02 has the potentiality to be applied in the treatment of the pollutant cyclohexylamine, and it could also be treated as a research material to study the degradation mechanism of cyclohexylamine.
不动杆菌属YT-02是从甜蜜素生产厂的活性污泥中分离出的革兰氏阴性菌，具有降解环己胺的能力。它被归类为不动杆菌属的成员，这是一种革兰氏阴性菌，与广东不动杆菌菌株1NM-4的16S rRNA基因序列同一性为99%。它能在22小时内降解10 mmol/L的环己胺。基于鉴定出的代谢产物，环己胺的代谢途径可推测为通过环己酮进行降解。本文给出了该菌株的基因组草图序列（染色体长度为2,993,647 bp）。我们进一步鉴定了编码参与环己胺氧化为环己酮以及随后环己酮氧化下游代谢途径的酶的基因。菌株YT-02有潜力应用于污染物环己胺的处理，也可作为研究环己胺降解机制的研究材料。
