Kurdziel Magdalena, Filek Maria, Łabanowska Maria
Faculty of Chemistry, Jagiellonian University, Kraków, Poland.
Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Kraków, Poland.
J Sci Food Agric. 2018 May;98(7):2607-2616. doi: 10.1002/jsfa.8753. Epub 2017 Nov 21.
UV irradiation has ionisation character and leads to the generation of reactive oxygen species (ROS). The destructive character of ROS was observed among others during interaction of cereal grains with ozone and was caused by changes in structures of biomolecules leading to the formation of stable organic radicals. That effect was more evident for stress sensitive genotypes. In this study we investigated the influence of UV irradiation on cereal grains originating from genotypes with different tolerance to oxidative stress.
Grains and their parts (endosperm, embryo and seed coat) of barley, wheat and oat were subjected to short-term UV irradiation. It was found that UV caused the appearance of various kinds of reactive species (O , H O ) and stable radicals (semiquinone, phenoxyl and carbon-centred). Simultaneously, lipid peroxidation occurred and the organic structure of Mn(II) and Fe(III) complexes become disturbed.
UV irradiation causes damage of main biochemical structures of plant tissues, the effect is more significant in sensitive genotypes. In comparison with ozone treatment, UV irradiation leads to stronger destruction of biomolecules in grains and their parts. It is caused by the high energy of UV light, facilitating easier breakage of molecular bonds in biochemical compounds. © 2017 Society of Chemical Industry.
紫外线照射具有电离特性，会导致活性氧（ROS）的产生。在谷物与臭氧相互作用的过程中观察到了ROS的破坏特性，这是由生物分子结构的变化导致稳定有机自由基的形成引起的。这种效应在对胁迫敏感的基因型中更为明显。在本研究中，我们研究了紫外线照射对源自对氧化应激具有不同耐受性的基因型的谷物的影响。
对大麦、小麦和燕麦的籽粒及其部分（胚乳、胚和种皮）进行了短期紫外线照射。发现紫外线会导致各种活性物质（O 、H O ）和稳定自由基（半醌、苯氧基和碳中心自由基）的出现。同时，发生了脂质过氧化，Mn（II）和Fe（III）配合物的有机结构受到干扰。
紫外线照射会导致植物组织主要生化结构的损伤，在敏感基因型中这种效应更为显著。与臭氧处理相比，紫外线照射会导致谷物及其部分中生物分子的更强破坏。这是由紫外线的高能量引起的，它更容易使生化化合物中的分子键断裂。©2017化学工业协会。
