Department of Urology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Department of Cellular and Organ Pathology, Graduate School of Medicine, Akita University, Akita, Japan.
Neurourol Urodyn. 2018 Feb;37(2):650-657. doi: 10.1002/nau.23365. Epub 2017 Oct 24.
To evaluate the significance of mast cell infiltration in interstitial cystitis (IC) by comparison with equally inflamed controls using a digital quantification technique.
Bladder biopsy specimens from 31 patients with Hunner type IC and 38 patients with non-Hunner type IC were analyzed. Bladder biopsy specimens from 37 patients without IC, including 19 non-specific chronic cystitis ("non-IC cystitis") specimens and 18 non-inflamed bladder ("normal bladder") specimens, were used as controls. Mast cell tryptase-, CD3-, CD20-, and CD138-immunoreactive cells were quantified using digital image analysis software to evaluate both mast cell and lymphoplasmacytic cell densities. Mast cell and lymphoplasmacytic cell densities were counted independently in the entire lamina propria and detrusor areas and compared among the four groups.
In the lamina propria, there were no significant differences in mast cell and lymphoplasmacytic cell densities between Hunner type IC and non-IC cystitis or between non-Hunner type IC and normal bladder specimens. In the detrusor, the mast cell densities were not significantly different among the four groups. Mast cell density was correlated with lymphoplasmacytic cell density, but not with clinical parameters.
Mast cell density is not significantly different between IC specimens and non-IC control specimens with a similar degree of background inflammation. The intensity of mast cell infiltration generally correlated with that of lymphoplasmacytic cells. We conclude that mast cell count is of no value in the differential diagnosis between IC and other etiologies.
通过与同样炎症的对照相比，使用数字量化技术评估肥大细胞浸润在间质性膀胱炎（IC）中的意义。
分析了 31 例 Hunner 型 IC 和 38 例非 Hunner 型 IC 患者的膀胱活检标本。还分析了 37 例无 IC 的患者的膀胱活检标本，包括 19 例非特异性慢性膀胱炎（“非 IC 膀胱炎”）标本和 18 例非炎症性膀胱（“正常膀胱”）标本，作为对照。使用数字图像分析软件定量评估肥大细胞和淋巴浆细胞密度，以评估肥大细胞和淋巴浆细胞密度。分别在整个固有层和逼尿肌区域独立计数肥大细胞和淋巴浆细胞密度，并在四组之间进行比较。
在固有层中，Hunner 型 IC 与非 IC 膀胱炎或非 Hunner 型 IC 与正常膀胱标本之间的肥大细胞和淋巴浆细胞密度没有显著差异。在逼尿肌中，四组之间的肥大细胞密度没有显著差异。肥大细胞密度与淋巴浆细胞密度相关，但与临床参数无关。
肥大细胞密度在具有相似背景炎症的 IC 标本和非 IC 对照标本之间没有显著差异。肥大细胞浸润的强度通常与淋巴浆细胞的强度相关。我们得出结论，肥大细胞计数在 IC 与其他病因的鉴别诊断中没有价值。
