白介素 1 和转化生长因子-β1 对大鼠肋软骨细胞的形态学、免疫细胞化学和生物化学研究
Morphological, Immunocytochemical, and Biochemical Studies of Rat Costal Chondrocytes Exposed to IL-1 and TGF-1.
机构信息
Department of Dermatology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
出版信息
J Healthc Eng. 2017;2017:9747264. doi: 10.1155/2017/9747264. Epub 2017 Jul 4.
This study was undertaken to determine the effects of IL-1 and TGF-1 on the expression of differentiation-associated genes in chondrocytes in vitro. Rat costal chondrocytes were exposed to different concentrations of IL-1 and TGF-1 for 48 h and tested for gene expression. IL-1 increased the expression of aggrecanase-1 and aggrecanase-2 and decreased the content of aggrecan and collagen II. Low concentration of TGF-1 decreased the expression of aggrecan and collagen II and increased the expression of aggrecanase-2. However, the level of aggrecanase-1 was significantly elevated in the presence of high concentration of TGF-1. IL-1 and TGF-1 show the ability to modulate the production of aggrecan and collagen II in chondrocytes in vitro.
本研究旨在确定 IL-1 和 TGF-1 对体外软骨细胞分化相关基因表达的影响。将大鼠肋软骨细胞暴露于不同浓度的 IL-1 和 TGF-1 中 48 小时，然后检测基因表达情况。结果表明，IL-1 增加了软骨素酶-1 和软骨素酶-2 的表达，降低了软骨素和 II 型胶原的含量。低浓度的 TGF-1 降低了软骨素和 II 型胶原的表达，增加了软骨素酶-2 的表达。然而，高浓度 TGF-1 存在时，软骨素酶-1 的水平显著升高。IL-1 和 TGF-1 表现出调节体外软骨细胞中软骨素和 II 型胶原产生的能力。
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